| RRC ID |
34507
|
| Author |
Lee S, Jia B, Pham BP, Shao Y, Kwak JM, Cheong GW.
|
| Title |
Architecture and characterization of sarcosine oxidase from Thermococcus kodakarensis KOD1.
|
| Journal |
Extremophiles
|
| Abstract |
Sarcosine oxidase (SOX) catalyzes the oxidation of the methyl group in sarcosine and transfer of the oxidized methyl group into the one-carbon metabolic pool. Here, we separately cloned and expressed α and β subunit of SOX from Thermococcus kodakarensis KOD1 (TkSOX) in Escherichia coli and the recombinant proteins were purified to homogeneity. Gel filtration chromatography and transmission electron microscopy analysis showed that the α subunit formed a dimeric structure and behaved as an NADH dehydrogenase; β subunit was a tetramer that had sarcosine oxidase and L: -proline dehydrogenase activity. The TkSOX complex assembled into the hetero-octameric (αβ)(4) form and had NADH dehydrogenase activity. Gold-label analysis indicated that α and β subunits were oriented in the alternative form. Based on these results, we suggested that TkSOX was a multifunctional enzyme and that each subunit and (αβ)(4) complex may separately exist as a function enzyme in different conditions.
|
| Volume |
16(1)
|
| Pages |
87-93
|
| Published |
2012-1-1
|
| DOI |
10.1007/s00792-011-0408-x
|
| PMID |
22083128
|
| MeSH |
Base Sequence
Biocatalysis
Chromatography, Gel
DNA Primers
Microscopy, Electron
Polymerase Chain Reaction
Sarcosine Oxidase / chemistry
Sarcosine Oxidase / metabolism*
Thermococcus / enzymology*
|
| IF |
2.462
|
| Times Cited |
9
|
|
WOS Category
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| General Microbes |
JCM 12380 |
