| RRC ID |
3456
|
| 著者 |
Hossain M, Shimizu S, Fujiwara H, Sakurai S, Iwami M.
|
| タイトル |
EcR expression in the prothoracicotropic hormone-producing neurosecretory cells of the Bombyx mori brain.
|
| ジャーナル |
FEBS J
|
| Abstract |
The steroid hormone 20-hydroxyecdysone (20E) initiates insect molting and metamorphosis through binding with a heterodimer of two nuclear receptors, the ecdysone receptor (EcR) and ultraspiracle (USP). Expression of the specific isoforms EcR-A and EcR-B1 governs steroid-induced responses in the developing cells of the silkworm Bombyx mori. Here, analysis of EcR-A and EcR-B1 expression during larval-pupal development showed that both genes were up-regulated by 20E in the B. mori brain. Whole-mount in situ hybridization and immunohistochemistry revealed that EcR-A and EcR-B1 mRNAs and proteins were exclusively located in two pairs of lateral neurosecretory cells in the larval brain known as the prothoracicotropic hormone (PTTH)- producing cells (PTPCs). In the pupal brain, EcR-A and EcR-B1 expression was detected in tritocerebral cells and optic lobe cells in addition to PTPCs. As PTTH controls ecdysone secretion by the prothoracic gland, these results indicate that 20E-responsive PTPCs are the master cells of insect metamorphosis.
|
| 巻・号 |
273(16)
|
| ページ |
3861-8
|
| 公開日 |
2006-8-1
|
| DOI |
10.1111/j.1742-4658.2006.05398.x
|
| PII |
EJB5398
|
| PMID |
16911531
|
| MeSH |
Animals
Bombyx / growth & development
Bombyx / physiology*
Brain / cytology
Brain / metabolism*
Ecdysterone / pharmacology
Gene Expression Regulation, Developmental*
Insect Hormones / metabolism*
Life Cycle Stages
Protein Isoforms / classification
Protein Isoforms / drug effects
Protein Isoforms / metabolism
Receptors, Steroid / classification
Receptors, Steroid / drug effects
Receptors, Steroid / metabolism*
Up-Regulation
|
| IF |
4.392
|
| 引用数 |
8
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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