RRC ID 34561
著者 Li J, Iwanami N, Hoa VQ, Furutani-Seiki M, Takahama Y.
タイトル Noninvasive intravital imaging of thymocyte dynamics in medaka.
ジャーナル J Immunol
Abstract In vivo imaging of thymocytes has not been accomplished due to their localization deep within opaque body and high susceptibility to surgical stress. To overcome these problems, medaka is useful because of transparency and ex-uterine development. We report the noninvasive detection of thymocytes in transgenic medaka that express fluorescent protein under the control of immature-lymphocyte-specific rag1. We show that lymphoid progenitor cells colonize the thymus primordium in an anterior-to-posterior orientation-specific manner, revealing that extrathymic anterior components guide prevascular thymus colonization. We also show that developing thymocytes acquire "random walk motility" along with the expression of Ag receptors and coreceptors, suggesting that thymocyte walking is initiated at the developmental stage for repertoire selection. Thus, transgenic medaka enables real-time intravital imaging of thymocytes without surgical invasion.
巻・号 179(3)
ページ 1605-15
公開日 2007-8-1
DOI 10.4049/jimmunol.179.3.1605
PII 179/3/1605
PMID 17641027
MeSH Animals Animals, Genetically Modified Cell Movement / immunology Genes, RAG-1 Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Homeodomain Proteins / biosynthesis Homeodomain Proteins / genetics Laser Scanning Cytometry Microscopy, Fluorescence Microscopy, Video Molecular Sequence Data Oryzias / embryology Oryzias / genetics Oryzias / immunology* T-Lymphocytes / cytology* T-Lymphocytes / metabolism* Thymus Gland / cytology Thymus Gland / embryology Thymus Gland / metabolism
IF 4.886
引用数 32
WOS 分野 IMMUNOLOGY
リソース情報
メダカ cab-Tg(rag1-egfp) (TG848) OK-Cab (MT830)