RRC ID 34567
Author Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P.
Title Effect of lentiviral vector overexpression α-calcitonin gene-related peptide on titanium implant osseointegration in α-CGRP-deficient mice.
Journal Bone
Abstract α-Calcitonin gene-related peptide (α-CGRP) plays a significant pathophysiological role in bone development, metabolism and remodeling around dental implants. However, the half-life of α-CGRP in plasma is only 10min, which affects its long-time application and an alternative approach should be developed to deliver α-CGRP over long periods of time. The aim of this study is to investigate whether a lentiviral α-CGRP overexpression vector system can express this target-gene longer at peri-implant sites, thus enhancing osseointegration. Animals were divided to the following groups: α-CGRP-/-, α-CGRP-/- with lentivirus transfection and α-CGRP+/+ mice. IVIS Spectrum imaging observations identified the successful transfection of α-CGRP around experimental implants inserted in the femurs at 5days after injection. Histomorphometrical analysis indicated an increase of bone-implant contact (BIC) at 1-month healing in the transfection group. Moreover, real-time RT-PCR and western blot results of bone-related markers Runx2, Osterix, and BSP levels elevated in lentivirus-transfected mice at 21days, compared to the untreated α-CGRP-/- mice. There was no significant difference between the transfection group and α-CGRP+/+ group. Further α-CGRP protein detection confirmed the persistent expression of this transgene at 21days post-operatively. These results suggest that this lentiviral vector system expresses α-CGRP in an effective, appropriate and sustained manner, which might have a potential application in enhancing titanium implant osseointegration.
Volume 94
Pages 135-140
Published 2017-1-1
DOI 10.1016/j.bone.2015.08.009
PII S8756-3282(15)00320-8
PMID 26265538
MeSH Animals Blotting, Western Calcitonin Gene-Related Peptide / deficiency* Calcitonin Gene-Related Peptide / genetics* Femur / pathology Genetic Vectors / metabolism* Green Fluorescent Proteins / metabolism Implants, Experimental* Lentivirus / metabolism* Mice Osseointegration / drug effects* Osteogenesis / drug effects Phenotype Real-Time Polymerase Chain Reaction Titanium / pharmacology* Transfection
IF 4.147
Times Cited 15
Mice RBRC04109