論文 - 詳細
| RRC ID | 34567 |
|---|---|
| 著者 | Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P. |
| タイトル | Effect of lentiviral vector overexpression α-calcitonin gene-related peptide on titanium implant osseointegration in α-CGRP-deficient mice. |
| ジャーナル | Bone |
| Abstract |
α-Calcitonin gene-related peptide (α-CGRP) plays a significant pathophysiological role in bone development, metabolism and remodeling around dental implants. However, the half-life of α-CGRP in plasma is only 10min, which affects its long-time application and an alternative approach should be developed to deliver α-CGRP over long periods of time. The aim of this study is to investigate whether a lentiviral α-CGRP overexpression vector system can express this target-gene longer at peri-implant sites, thus enhancing osseointegration. Animals were divided to the following groups: α-CGRP-/-, α-CGRP-/- with lentivirus transfection and α-CGRP+/+ mice. IVIS Spectrum imaging observations identified the successful transfection of α-CGRP around experimental implants inserted in the femurs at 5days after injection. Histomorphometrical analysis indicated an increase of bone-implant contact (BIC) at 1-month healing in the transfection group. Moreover, real-time RT-PCR and western blot results of bone-related markers Runx2, Osterix, and BSP levels elevated in lentivirus-transfected mice at 21days, compared to the untreated α-CGRP-/- mice. There was no significant difference between the transfection group and α-CGRP+/+ group. Further α-CGRP protein detection confirmed the persistent expression of this transgene at 21days post-operatively. These results suggest that this lentiviral vector system expresses α-CGRP in an effective, appropriate and sustained manner, which might have a potential application in enhancing titanium implant osseointegration. |
| 巻・号 | 94 |
| ページ | 135-140 |
| 公開日 | 2017-1-1 |
| DOI | 10.1016/j.bone.2015.08.009 |
| PII | S8756-3282(15)00320-8 |
| PMID | 26265538 |
| MeSH | Animals Blotting, Western Calcitonin Gene-Related Peptide / deficiency* Calcitonin Gene-Related Peptide / genetics* Femur / pathology Genetic Vectors / metabolism* Green Fluorescent Proteins / metabolism Implants, Experimental* Lentivirus / metabolism* Mice Osseointegration / drug effects* Osteogenesis / drug effects Phenotype Real-Time Polymerase Chain Reaction Titanium / pharmacology* Transfection |
| IF | 4.147 |
| 引用数 | 15 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC04109 |