| RRC ID |
34567
|
| 著者 |
Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P.
|
| タイトル |
Effect of lentiviral vector overexpression α-calcitonin gene-related peptide on titanium implant osseointegration in α-CGRP-deficient mice.
|
| ジャーナル |
Bone
|
| Abstract |
α-Calcitonin gene-related peptide (α-CGRP) plays a significant pathophysiological role in bone development, metabolism and remodeling around dental implants. However, the half-life of α-CGRP in plasma is only 10min, which affects its long-time application and an alternative approach should be developed to deliver α-CGRP over long periods of time. The aim of this study is to investigate whether a lentiviral α-CGRP overexpression vector system can express this target-gene longer at peri-implant sites, thus enhancing osseointegration. Animals were divided to the following groups: α-CGRP-/-, α-CGRP-/- with lentivirus transfection and α-CGRP+/+ mice. IVIS Spectrum imaging observations identified the successful transfection of α-CGRP around experimental implants inserted in the femurs at 5days after injection. Histomorphometrical analysis indicated an increase of bone-implant contact (BIC) at 1-month healing in the transfection group. Moreover, real-time RT-PCR and western blot results of bone-related markers Runx2, Osterix, and BSP levels elevated in lentivirus-transfected mice at 21days, compared to the untreated α-CGRP-/- mice. There was no significant difference between the transfection group and α-CGRP+/+ group. Further α-CGRP protein detection confirmed the persistent expression of this transgene at 21days post-operatively. These results suggest that this lentiviral vector system expresses α-CGRP in an effective, appropriate and sustained manner, which might have a potential application in enhancing titanium implant osseointegration.
|
| 巻・号 |
94
|
| ページ |
135-140
|
| 公開日 |
2017-1-1
|
| DOI |
10.1016/j.bone.2015.08.009
|
| PII |
S8756-3282(15)00320-8
|
| PMID |
26265538
|
| MeSH |
Animals
Blotting, Western
Calcitonin Gene-Related Peptide / deficiency*
Calcitonin Gene-Related Peptide / genetics*
Femur / pathology
Genetic Vectors / metabolism*
Green Fluorescent Proteins / metabolism
Implants, Experimental*
Lentivirus / metabolism*
Mice
Osseointegration / drug effects*
Osteogenesis / drug effects
Phenotype
Real-Time Polymerase Chain Reaction
Titanium / pharmacology*
Transfection
|
| IF |
4.147
|
| 引用数 |
15
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| 実験動物マウス |
RBRC04109 |
