RRC ID 3462
Author Katsuma S, Daimon T, Mita K, Shimada T.
Title Lepidopteran ortholog of Drosophila breathless is a receptor for the baculovirus fibroblast growth factor.
Journal J. Virol.
Abstract The Bombyx mori nucleopolyhedrovirus (BmNPV) encodes a gene homologous to the mammalian fibroblast growth factor (FGF) family. We report the cloning of B. mori and Spodoptera frugiperda orthologous genes (Bmbtl and Sfbtl, respectively) of Drosophila melanogaster breathless (btl) encoding a receptor for Branchless/FGF and show that these genes encode the receptor for a baculovirus-encoded FGF (vFGF). Sequence analysis showed that BmBtl is composed of 856 amino acid residues, which potentially encodes a 97.3-kDa polypeptide and shares structural features and sequence similarities with the FGF receptor family. Reverse transcription-PCR experiments showed that Bmbtl was abundantly expressed in the trachea and midgut in B. mori larvae, with moderate expression observed in the hemocytes and the B. mori cultured cell line BmN. We generated Sf-9 cells that stably expressed His-tagged BmBtl. Western blot analysis revealed that BmBtl was an approximately 110-kDa protein. Immunoprecipitation experiments showed that BmNPV vFGF markedly phosphorylated BmBtl in Sf-9 cells. In addition, we found that BmBtl overexpression enhanced the migration activity for BmNPV vFGF. Furthermore, we generated Sf-9 cells in which Sfbtl was knocked down by transfection with double-strand RNA-expressing plasmids. In these cells, cell motility triggered by vFGF was markedly reduced. These results strongly suggest that the Btl orthologs, BmBtl and SfBtl, are the receptors for vFGF, which mediate vFGF-induced host cell chemotaxis.
Volume 80(11)
Pages 5474-81
Published 2006-6
DOI 10.1128/JVI.00248-06
PII 80/11/5474
PMID 16699027
PMC PMC1472154
MeSH Animals Baculoviridae / chemistry* Bombyx / virology* Cells, Cultured Drosophila Proteins / metabolism* Fibroblast Growth Factors / chemistry Fibroblast Growth Factors / genetics Fibroblast Growth Factors / metabolism* Lepidoptera / virology Molecular Sequence Data Protein-Tyrosine Kinases / metabolism* Receptors, Fibroblast Growth Factor / metabolism*
IF 4.368
Times Cited 33
WOS Category VIROLOGY
Resource
Silkworms p50 EST