| RRC ID |
3469
|
| Author |
Daimon T, Katsuma S, Kang W, Shimada T.
|
| Title |
Comparative studies of Bombyx mori nucleopolyhedrovirus chitinase and its host ortholog, BmChi-h.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Baculovirus-encoded chitinases (V-CHIAs) were first proposed to be acquired from a bacterium via horizontal gene transfer. However, we have recently reported that lepidopteran hosts also encode v-chiA orthologs. Here we describe comparative studies of Bombyx mori nucleopolyhedrovirus (BmNPV) chitinase and its host ortholog, BmChi-h. We constructed recombinant BmNPVs in which native and modified forms of BmChi-h were driven under the polyhedrin promoter and the authentic v-chiA was deleted. Western blot analysis indicated that BmCHI-h was rapidly secreted from virus-infected BmN cells whereas BmNPV CHIA was localized within the virus-infected cells; probably because of the presence of a C-terminal endoplasmic reticulum retention motif on BmNPV CHIA. Enzymological studies showed that BmNPV CHIA was able to retain much higher chitinolytic activity under alkaline conditions. For B. mori larvae infected with v-chiA-deleted BmNPV, the terminal liquefaction of dead larvae and the activation of baculovirus-encoded cysteine protease were not observed, and the introduction of BmChi-h did not rescue these defects. Our findings show that BmNPV chiA possesses unique features that are not shared by host orthologs, which may reflect functional specialization of baculovirus chitinases.
|
| Volume |
345(2)
|
| Pages |
825-33
|
| Published |
2006-6-30
|
| DOI |
10.1016/j.bbrc.2006.04.112
|
| PII |
S0006-291X(06)00912-0
|
| PMID |
16701557
|
| MeSH |
Animals
Baculoviridae / enzymology*
Baculoviridae / genetics
Baculoviridae / pathogenicity
Baculoviridae / physiology
Blotting, Western
Bombyx / virology*
Cells, Cultured
Chitinases / genetics
Chitinases / metabolism*
Cloning, Molecular
Cysteine Endopeptidases / genetics
Cysteine Endopeptidases / metabolism
Nucleopolyhedroviruses / enzymology*
Nucleopolyhedroviruses / genetics
Nucleopolyhedroviruses / growth & development
Occlusion Body Matrix Proteins
Promoter Regions, Genetic
Viral Proteins / genetics
Viral Proteins / metabolism
Viral Structural Proteins
|
| IF |
2.985
|
| Times Cited |
19
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
EST clone wdS20876 ? |
