| RRC ID |
34778
|
| Author |
Ogawa Y, Eto A, Miyake C, Tsuchida N, Miyake H, Takaku Y, Hagiwara H, Oishi K.
|
| Title |
Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice.
|
| Journal |
PLoS One
|
| Abstract |
Neural crest (NC) cells are a migratory, multipotent cell population that arises at the neural plate border, and migrate from the dorsal neural tube to their target tissues, where they differentiate into various cell types. Abnormal development of NC cells can result in severe congenital birth defects. Because only a limited number of cells can be obtained from an embryo, mechanistic studies are difficult to perform with directly isolated NC cells. Protein zero (P0) is expressed by migrating NC cells during the early embryonic period. In the P0-Cre;Z/EG transgenic mouse, transient activation of the P0 promoter induces Cre-mediated recombination, indelibly tagging NC-derived cells with enhanced green fluorescent protein (EGFP). Induced pluripotent stem cell (iPSC) technology offers new opportunities for both mechanistic studies and development of stem cell-based therapies. Here, we report the generation of iPSCs from the P0-Cre;Z/EG mouse. P0-Cre;Z/EG mouse-derived iPSCs (P/G-iPSCs) exhibited pluripotent stem cell properties. In lineage-directed differentiation studies, P/G-iPSCs were efficiently differentiated along the neural lineage while expressing EGFP. These results suggest that P/G-iPSCs are useful to study NC development and NC-associated diseases.
|
| Volume |
10(9)
|
| Pages |
e0138620
|
| Published |
2015-1-1
|
| DOI |
10.1371/journal.pone.0138620
|
| PII |
PONE-D-15-29170
|
| PMID |
26382630
|
| PMC |
PMC4575135
|
| MeSH |
Animals
Cell Differentiation*
Cell Lineage / genetics
Embryo, Mammalian / cytology
Embryo, Mammalian / embryology
Embryo, Mammalian / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Immunohistochemistry
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Lac Operon / genetics
Mice, Inbred BALB C
Mice, Nude
Mice, Transgenic
Microscopy, Confocal
Microscopy, Fluorescence
Multipotent Stem Cells / cytology*
Multipotent Stem Cells / metabolism
Myelin P0 Protein / genetics
Myelin P0 Protein / metabolism
Neural Crest / cytology*
Neural Crest / embryology
Neural Crest / metabolism
|
| IF |
2.74
|
| Times Cited |
1
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Mice |
RBRC01459 |
