論文 - 詳細
| RRC ID | 34789 |
|---|---|
| 著者 | Yoshida S, Yasuda M, Miyashita H, Ogawa Y, Yoshida T, Matsuzaki Y, Tsubota K, Okano H, Shimmura S. |
| タイトル | Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Application of induced pluripotent stem (iPS) cells in regenerative medicine will bypass ethical issues associated with use of embryonic stem cells. In addition, patient-specific IPS cells can be useful to elucidate the pathophysiology of genetic disorders, drug screening, and tailor-made medicine. However, in order to apply iPS cells to mitotic tissue, induction of tissue stem cells that give rise to progeny of the target organ is required. METHODOLOGY/PRINCIPAL FINDINGS:We induced stratified epithelial cells from mouse iPS cells by co-culture with PA6 feeder cells (SDIA-method) with use of BMP4. Clusters of cells positive for the differentiation markers KRT1 or KRT12 were observed in KRT14-positive colonies. We successfully cloned KRT14 and p63 double-positive stratified epithelial progenitor cells from iPS-derived epithelial cells, which formed stratified epithelial sheets consisting of five- to six-polarized epithelial cells in vitro. When these clonal cells were cultured on denuded mouse corneas, a robust stratified epithelial layer was observed with physiological cell polarity including high levels of E-cadherin, p63 and K15 expression in the basal layer and ZO-1 in the superficial layer, recapitulating the apico-basal polarity of the epithelium in vivo. CONCLUSIONS/SIGNIFICANCE:These results suggest that KRT14 and p63 double-positive epithelial progenitor cells can be cloned from iPS cells in order to produce polarized multilayer epithelial cell sheets. |
| 巻・号 | 6(12) |
| ページ | e28856 |
| 公開日 | 2011-1-1 |
| DOI | 10.1371/journal.pone.0028856 |
| PII | PONE-D-11-13236 |
| PMID | 22174914 |
| PMC | PMC3235161 |
| MeSH | Animals Bone Morphogenetic Protein 4 / pharmacology Cell Culture Techniques / methods* Cell Differentiation / drug effects Cell Proliferation / drug effects Cells, Cultured Clone Cells Cornea / cytology Epidermal Cells Epidermis / drug effects Epidermis / metabolism Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / ultrastructure Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / metabolism Mice Stem Cells / cytology* Stem Cells / drug effects Stem Cells / metabolism Time Factors Tissue Engineering |
| IF | 2.74 |
| 引用数 | 18 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | CSII-EF-mRFP1 (RDB06387) |