RRC ID 34794
Author Dohra H, Fujishima M, Suzuki H.
Title Analysis of amino acid and codon usage in Paramecium bursaria.
Journal FEBS Lett.
Abstract The ciliate Paramecium bursaria harbors the green-alga Chlorella symbionts. We reassembled the P. bursaria transcriptome to minimize falsely fused transcripts, and investigated amino acid and codon usage using the transcriptome data. Surface proteins preferentially use smaller amino acid residues like cysteine. Unusual synonymous codon and amino acid usage in highly expressed genes can reflect a balance between translational selection and other factors. A correlation of gene expression level with synonymous codon or amino acid usage is emphasized in genes down-regulated in symbiont-bearing cells compared to symbiont-free cells. Our results imply that the selection is associated with P. bursaria-Chlorella symbiosis.
Volume 589(20 Pt B)
Pages 3113-8
Published 2015-10-7
DOI 10.1016/j.febslet.2015.08.033
PII S0014-5793(15)00733-4
PMID 26341535
MeSH Amino Acids / genetics* Amino Acids / metabolism Chlorella / physiology Codon / genetics* Gene Expression Profiling / methods Host-Pathogen Interactions Paramecium / genetics* Paramecium / metabolism Paramecium / microbiology Protein Biosynthesis Protozoan Proteins / genetics Protozoan Proteins / metabolism Symbiosis Transcriptome*
IF 2.675
Times Cited 1
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Paramecium P. bursaria strains Yad1w and Yad1g1N