論文 - 詳細
| RRC ID | 34801 |
|---|---|
| 著者 | Inoue K, Oikawa M, Kamimura S, Ogonuki N, Nakamura T, Nakano T, Abe K, Ogura A. |
| タイトル | Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer. |
| ジャーナル | Sci Rep |
| Abstract |
Although mammalian cloning by somatic cell nuclear transfer (SCNT) has been established in various species, the low developmental efficiency has hampered its practical applications. Treatment of SCNT-derived embryos with histone deacetylase (HDAC) inhibitors can improve their development, but the underlying mechanism is still unclear. To address this question, we analysed gene expression profiles of SCNT-derived 2-cell mouse embryos treated with trichostatin A (TSA), a potent HDAC inhibitor that is best used for mouse cloning. Unexpectedly, TSA had no effect on the numbers of aberrantly expressed genes or the overall gene expression pattern in the embryos. However, in-depth investigation by gene ontology and functional analyses revealed that TSA treatment specifically improved the expression of a small subset of genes encoding transcription factors and their regulatory factors, suggesting their positive involvement in de novo RNA synthesis. Indeed, introduction of one of such transcription factors, Spi-C, into the embryos at least partially mimicked the TSA-induced improvement in embryonic development by activating gene networks associated with transcriptional regulation. Thus, the effects of TSA treatment on embryonic gene expression did not seem to be stochastic, but more specific than expected, targeting genes that direct development and trigger zygotic genome activation at the 2-cell stage. |
| 巻・号 | 5 |
| ページ | 10127 |
| 公開日 | 2015-5-14 |
| DOI | 10.1038/srep10127 |
| PII | srep10127 |
| PMID | 25974394 |
| PMC | PMC4431350 |
| MeSH | Animals DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics Embryo Transfer / methods Embryo, Mammalian / embryology Embryonic Development / drug effects* Female Gene Expression / drug effects Gene Expression Profiling Gene Expression Regulation, Developmental / drug effects Histone Deacetylase Inhibitors / pharmacology* Hydroxamic Acids / pharmacology* Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Inbred ICR Molecular Sequence Data Nuclear Transfer Techniques* Oligonucleotide Array Sequence Analysis Transcription Factors / biosynthesis* Transcription Factors / genetics Transcription, Genetic / genetics |
| IF | 3.998 |
| 引用数 | 18 |
| WOS 分野 | REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pcDNA4 Myc-HisA-Spic (RDB13502) pcDNA4 Myc-HisA-Spic-GFP (RDB13503). |