RRC ID 34824
Author Igawa T, Watanabe A, Suzuki A, Kashiwagi A, Kashiwagi K, Noble A, Guille M, Simpson DE, Horb ME, Fujii T, Sumida M.
Title Inbreeding Ratio and Genetic Relationships among Strains of the Western Clawed Frog, Xenopus tropicalis.
Journal PLoS ONE
Abstract The Western clawed frog, Xenopus tropicalis, is a highly promising model amphibian, especially in developmental and physiological research, and as a tool for understanding disease. It was originally found in the West African rainforest belt, and was introduced to the research community in the 1990s. The major strains thus far known include the Nigerian and Ivory Coast strains. However, due to its short history as an experimental animal, the genetic relationship among the various strains has not yet been clarified, and establishment of inbred strains has not yet been achieved. Since 2003 the Institute for Amphibian Biology (IAB), Hiroshima University has maintained stocks of multiple X. tropicalis strains and conducted consecutive breeding as part of the National BioResource Project. In the present study we investigated the inbreeding ratio and genetic relationship of four inbred strains at IAB, as well as stocks from other institutions, using highly polymorphic microsatellite markers and mitochondrial haplotypes. Our results show successive reduction of heterozygosity in the genome of the IAB inbred strains. The Ivory Coast strains clearly differed from the Nigerian strains genetically, and three subgroups were identified within both the Nigerian and Ivory Coast strains. It is noteworthy that the Ivory Coast strains have an evolutionary divergent genetic background. Our results serve as a guide for the most effective use of X. tropicalis strains, and the long-term maintenance of multiple strains will contribute to further research efforts.
Volume 10(7)
Pages e0133963
Published 2015
DOI 10.1371/journal.pone.0133963
PII PONE-D-14-54578
PMID 26222540
PMC PMC4519292
MeSH Animals Chromosome Mapping Female Genetic Markers / genetics Genome, Mitochondrial / genetics Genotyping Techniques Inbreeding* Male Microsatellite Repeats / genetics Phylogeny Polymorphism, Genetic Species Specificity Xenopus / genetics*
IF 2.766
Times Cited 2
WOS Category GENETICS & HEREDITY
Resource
Xenopus tropicalis Ivory Coast Nigerian Golden