RRC ID 34830
Author Tsuyama J, Bunt J, Richards LJ, Iwanari H, Mochizuki Y, Hamakubo T, Shimazaki T, Okano H.
Title MicroRNA-153 Regulates the Acquisition of Gliogenic Competence by Neural Stem Cells.
Journal Stem Cell Reports
Abstract Mammalian neural stem/progenitor cells (NSPCs) sequentially generate neurons and glia during CNS development. Here we identified miRNA-153 (miR-153) as a modulator of the temporal regulation of NSPC differentiation. Overexpression (OE) of miR-153 delayed the onset of astrogliogenesis and maintained NSPCs in an undifferentiated state in vitro and in the developing cortex. The transcription factors nuclear factor I (NFI) A and B, essential regulators of the initiation of gliogenesis, were found to be targets of miR-153. Inhibition of miR-153 in early neurogenic NSPCs induced precocious gliogenesis, whereas NFIA/B overexpression rescued the anti-gliogenic phenotypes induced by miR-153 OE. Our results indicate that miR-mediated fine control of NFIA/B expression is important in the molecular networks that regulate the acquisition of gliogenic competence by NSPCs in the developing CNS.
Volume 5(3)
Pages 365-77
Published 2015-9-8
DOI 10.1016/j.stemcr.2015.06.006
PII S2213-6711(15)00187-3
PMID 26212661
PMC PMC4618452
MeSH Animals Cell Differentiation / physiology* Cerebral Cortex / cytology Cerebral Cortex / metabolism* Mice MicroRNAs / genetics MicroRNAs / metabolism* NFI Transcription Factors / genetics NFI Transcription Factors / metabolism Neural Stem Cells / cytology Neural Stem Cells / metabolism* Neuroglia / cytology Neuroglia / metabolism*
IF 6.537
Times Cited 4
DNA material pCAGGS-LIF (RDB09182) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)