RRC ID 34843
Author Suzuki T, Nakano-Ikegaya M, Yabukami-Okuda H, de Hoon M, Severin J, Saga-Hatano S, Shin JW, Kubosaki A, Simon C, Hasegawa Y, Hayashizaki Y, Suzuki H.
Title Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts.
Journal PLoS One
Abstract Transcriptional regulatory networks (TRN) control the underlying mechanisms behind cellular functions and they are defined by a set of core transcription factors regulating cascades of peripheral genes. Here we report SPI1, CEBPA, MNDA and IRF8 as core transcription factors of monocyte TRN and demonstrate functional inductions of phagocytosis, inflammatory response and chemotaxis activities in human dermal fibroblasts. The Gene Ontology and KEGG pathway analyses also revealed notable representation of genes involved in immune response and endocytosis in fibroblasts. Moreover, monocyte TRN-inducers triggered multiple monocyte-specific genes based on the transcription factor motif response analysis and suggest that complex cellular TRNs are uniquely amenable to elicit cell-specific functions in unrelated cell types.
Volume 7(3)
Pages e33474
Published 2012-1-1
DOI 10.1371/journal.pone.0033474
PII PONE-D-11-11286
PMID 22428058
PMC PMC3302774
MeSH Chemotaxis / immunology DNA Primers / genetics Data Mining Fibroblasts / cytology* Flow Cytometry Gene Expression Profiling Gene Expression Regulation / immunology* Gene Regulatory Networks / immunology* Genetic Vectors / genetics Humans Lentivirus Lipopolysaccharides Microarray Analysis Monocytes / cytology* Monocytes / immunology Monocytes / metabolism Phagocytosis / immunology Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Skin / cytology Transcription Factors / immunology* Transcription Factors / metabolism
IF 2.776
Times Cited 23
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) CSII-CMV-RfA-IRES2-Venus (RDB04388) CSII-EF-RfA (RDB04387).
Human and Animal Cells NB1RGB(RCB0222)