RRC ID 34843
著者 Suzuki T, Nakano-Ikegaya M, Yabukami-Okuda H, de Hoon M, Severin J, Saga-Hatano S, Shin JW, Kubosaki A, Simon C, Hasegawa Y, Hayashizaki Y, Suzuki H.
タイトル Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts.
ジャーナル PLoS One
Abstract Transcriptional regulatory networks (TRN) control the underlying mechanisms behind cellular functions and they are defined by a set of core transcription factors regulating cascades of peripheral genes. Here we report SPI1, CEBPA, MNDA and IRF8 as core transcription factors of monocyte TRN and demonstrate functional inductions of phagocytosis, inflammatory response and chemotaxis activities in human dermal fibroblasts. The Gene Ontology and KEGG pathway analyses also revealed notable representation of genes involved in immune response and endocytosis in fibroblasts. Moreover, monocyte TRN-inducers triggered multiple monocyte-specific genes based on the transcription factor motif response analysis and suggest that complex cellular TRNs are uniquely amenable to elicit cell-specific functions in unrelated cell types.
巻・号 7(3)
ページ e33474
公開日 2012-1-1
DOI 10.1371/journal.pone.0033474
PII PONE-D-11-11286
PMID 22428058
PMC PMC3302774
MeSH Chemotaxis / immunology DNA Primers / genetics Data Mining Fibroblasts / cytology* Flow Cytometry Gene Expression Profiling Gene Expression Regulation / immunology* Gene Regulatory Networks / immunology* Genetic Vectors / genetics Humans Lentivirus Lipopolysaccharides Microarray Analysis Monocytes / cytology* Monocytes / immunology Monocytes / metabolism Phagocytosis / immunology Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Skin / cytology Transcription Factors / immunology* Transcription Factors / metabolism
IF 2.74
引用数 23
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
遺伝子材料 pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) CSII-CMV-RfA-IRES2-Venus (RDB04388) CSII-EF-RfA (RDB04387).
ヒト・動物細胞 NB1RGB(RCB0222)