RRC ID 34849
Author Asano T, Ishizua T, Yawo H.
Title Optically controlled contraction of photosensitive skeletal muscle cells.
Journal Biotechnol Bioeng
Abstract As the skeletal muscle cell is an efficient force transducer, it has been incorporated in bio-microdevices using electrical field stimulation for generating contractile patterns. To improve both the spatial and temporal resolutions, we made photosensitive skeletal muscle cells from murine C2C12 myoblasts, which express channelrhodopsin-2 (ChR2), one of archaea-type rhodopsins derived from green algae Chlamydomonas reinhardtii. The cloned ChR2-expressing C2C12 myoblasts were made and fused with untransfected C2C12 to form multinucleated myotubes. The maturation of myotubes was facilitated by electrical field stimulation. Blue LED light pulse depolarized the membrane potential of a ChR2-expressing myotube and eventually evoked an action potential. It also induced a twitch-like contraction in a concurrent manner. A contraction pattern was thus made with a given pattern of LED pulses. This technique would have many applications in the bioengineering field, such as wireless drive of muscle-powered actuators/microdevices.
Volume 109(1)
Pages 199-204
Published 2012-1-1
DOI 10.1002/bit.23285
PMID 21809334
MeSH Animals Carrier Proteins / genetics Carrier Proteins / metabolism* Light* Membrane Potentials / radiation effects Mice Mice, Transgenic Muscle Contraction* Muscle Fibers, Skeletal / physiology* Muscle Fibers, Skeletal / radiation effects* Muscle, Skeletal / physiology* Myoblasts / physiology* Recombinant Proteins / genetics Recombinant Proteins / metabolism
IF 4.002
Times Cited 32
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Human and Animal Cells C2C12(RCB0987)