Reference - Detail
| RRC ID | 34868 |
|---|---|
| Author | Clavier A, Ruby V, Rincheval-Arnold A, Mignotte B, Guénal I. |
| Title | The Drosophila retinoblastoma protein, Rbf1, induces a Debcl- and Drp1-dependent mitochondrial apoptosis. |
| Journal | J Cell Sci |
| Abstract |
In accordance with its tumor suppressor role, the retinoblastoma protein pRb can ensure pro-apoptotic functions. Rbf1, the Drosophila homolog of Rb, also displays a pro-apoptotic activity in proliferative cells. We have previously shown that the Rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. Buffy often acts in an opposite manner to Debcl, the other Drosophila Bcl-2-family protein. Both proteins can localize at the mitochondrion, but the way they control apoptosis still remains unclear. Here, we demonstrate that Debcl and the pro-fission gene Drp1 are necessary downstream of Buffy to trigger a mitochondrial fragmentation during Rbf1-induced apoptosis. Interestingly, Rbf1-induced apoptosis leads to a Debcl- and Drp1-dependent reactive oxygen species production, which in turn activates the Jun Kinase pathway to trigger cell death. Moreover, we show that Debcl and Drp1 can interact and that Buffy inhibits this interaction. Notably, Debcl modulates Drp1 mitochondrial localization during apoptosis. These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis, and shed light on a link between Rbf1 and mitochondrial dynamics in vivo. |
| Volume | 128(17) |
| Pages | 3239-49 |
| Published | 2015-9-1 |
| DOI | 10.1242/jcs.169896 |
| PII | jcs.169896 |
| PMID | 26208635 |
| MeSH | Animals Apoptosis / physiology* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Mitochondria / genetics Mitochondria / metabolism* Protein Binding Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism Retinoblastoma Protein Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 4.573 |
| Times Cited | 12 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 4353R-2 |