論文 - 詳細
| RRC ID | 34881 |
|---|---|
| 著者 | Bou Sleiman MS, Osman D, Massouras A, Hoffmann AA, Lemaitre B, Deplancke B. |
| タイトル | Genetic, molecular and physiological basis of variation in Drosophila gut immunocompetence. |
| ジャーナル | Nat Commun |
| Abstract |
Gut immunocompetence involves immune, stress and regenerative processes. To investigate the determinants underlying inter-individual variation in gut immunocompetence, we perform enteric infection of 140 Drosophila lines with the entomopathogenic bacterium Pseudomonas entomophila and observe extensive variation in survival. Using genome-wide association analysis, we identify several novel immune modulators. Transcriptional profiling further shows that the intestinal molecular state differs between resistant and susceptible lines, already before infection, with one transcriptional module involving genes linked to reactive oxygen species (ROS) metabolism contributing to this difference. This genetic and molecular variation is physiologically manifested in lower ROS activity, lower susceptibility to ROS-inducing agent, faster pathogen clearance and higher stem cell activity in resistant versus susceptible lines. This study provides novel insights into the determinants underlying population-level variability in gut immunocompetence, revealing how relatively minor, but systematic genetic and transcriptional variation can mediate overt physiological differences that determine enteric infection susceptibility. |
| 巻・号 | 6 |
| ページ | 7829 |
| 公開日 | 2015-7-27 |
| DOI | 10.1038/ncomms8829 |
| PII | ncomms8829 |
| PMID | 26213329 |
| PMC | PMC4525169 |
| MeSH | Animals Bacterial Load Drosophila Proteins / genetics Drosophila Proteins / immunology* Drosophila melanogaster / genetics Drosophila melanogaster / immunology* Drosophila melanogaster / physiology Gene Expression Profiling Gene Expression Regulation Genome-Wide Association Study Immunocompetence / genetics Immunocompetence / immunology* Immunocompetence / physiology Intestinal Mucosa / metabolism Intestines / immunology* Polymorphism, Single Nucleotide Pseudomonas / immunology Pseudomonas Infections / genetics Pseudomonas Infections / immunology* Pseudomonas Infections / mortality Reactive Oxygen Species / immunology Real-Time Polymerase Chain Reaction Sequence Analysis, RNA |
| IF | 12.121 |
| 引用数 | 26 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 31 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 4007R-2 4007R-3 |