論文 - 詳細
| RRC ID | 3508 |
|---|---|
| 著者 | Shiomi K, Fujiwara Y, Yasukochi Y, Kajiura Z, Nakagaki M, Yaginuma T. |
| タイトル | The Pitx homeobox gene in Bombyx mori: regulation of DH-PBAN neuropeptide hormone gene expression. |
| ジャーナル | Mol Cell Neurosci |
| Abstract |
The diapause hormone-pheromone biosynthesis activating neuropeptide gene, DH-PBAN, is expressed exclusively in seven pairs of DH-PBAN-producing neurosecretory cells (DHPCs) on the terminally differentiated processes of the subesophageal ganglion (SG). To help reveal the regulatory mechanisms of cell-specific DH-PBAN expression, we identified a cis-regulatory element that regulates expression in DHPCs using the recombinant AcNPV-mediated gene transfer system and a gel-mobility shift assay. Bombyx mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds this element and activates DH-PBAN expression. The BmPitx was expressed in various tissues, including DHPCs in the SG. Suppression of DH-PBAN expression by silencing of the BmPitx successfully induced non-diapaused eggs from a diapause egg producer. To the best of our knowledge, this report is the first to identify a neuropeptide-encoding gene as a target of the Pitx transcriptional regulator in invertebrates. Thus, it is tempting to speculate that functional conservation of Pitx family members on neuropeptide gene expression occurs through a "combinational code mechanism" in both vertebrate and invertebrate in neuroendocrine systems. |
| 巻・号 | 34(2) |
| ページ | 209-18 |
| 公開日 | 2007-2-1 |
| DOI | 10.1016/j.mcn.2006.10.015 |
| PII | S1044-7431(06)00239-9 |
| PMID | 17166739 |
| MeSH | Animals Bombyx / genetics* Cloning, Molecular Gene Expression Regulation / physiology* Genes, Homeobox / physiology* Green Fluorescent Proteins / metabolism Insect Proteins / metabolism* Larva Molecular Sequence Data Neuropeptides / metabolism* Protein Precursors / metabolism* RNA Interference / physiology Reverse Transcriptase Polymerase Chain Reaction / methods |
| IF | 3.182 |
| 引用数 | 16 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | |