RRC ID |
3508
|
著者 |
Shiomi K, Fujiwara Y, Yasukochi Y, Kajiura Z, Nakagaki M, Yaginuma T.
|
タイトル |
The Pitx homeobox gene in Bombyx mori: regulation of DH-PBAN neuropeptide hormone gene expression.
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ジャーナル |
Mol Cell Neurosci
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Abstract |
The diapause hormone-pheromone biosynthesis activating neuropeptide gene, DH-PBAN, is expressed exclusively in seven pairs of DH-PBAN-producing neurosecretory cells (DHPCs) on the terminally differentiated processes of the subesophageal ganglion (SG). To help reveal the regulatory mechanisms of cell-specific DH-PBAN expression, we identified a cis-regulatory element that regulates expression in DHPCs using the recombinant AcNPV-mediated gene transfer system and a gel-mobility shift assay. Bombyx mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds this element and activates DH-PBAN expression. The BmPitx was expressed in various tissues, including DHPCs in the SG. Suppression of DH-PBAN expression by silencing of the BmPitx successfully induced non-diapaused eggs from a diapause egg producer. To the best of our knowledge, this report is the first to identify a neuropeptide-encoding gene as a target of the Pitx transcriptional regulator in invertebrates. Thus, it is tempting to speculate that functional conservation of Pitx family members on neuropeptide gene expression occurs through a "combinational code mechanism" in both vertebrate and invertebrate in neuroendocrine systems.
|
巻・号 |
34(2)
|
ページ |
209-18
|
公開日 |
2007-2-1
|
DOI |
10.1016/j.mcn.2006.10.015
|
PII |
S1044-7431(06)00239-9
|
PMID |
17166739
|
MeSH |
Animals
Bombyx / genetics*
Cloning, Molecular
Gene Expression Regulation / physiology*
Genes, Homeobox / physiology*
Green Fluorescent Proteins / metabolism
Insect Proteins / metabolism*
Larva
Molecular Sequence Data
Neuropeptides / metabolism*
Protein Precursors / metabolism*
RNA Interference / physiology
Reverse Transcriptase Polymerase Chain Reaction / methods
|
IF |
3.182
|
引用数 |
16
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
カイコ |
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