RRC ID 3508
著者 Shiomi K, Fujiwara Y, Yasukochi Y, Kajiura Z, Nakagaki M, Yaginuma T.
タイトル The Pitx homeobox gene in Bombyx mori: regulation of DH-PBAN neuropeptide hormone gene expression.
ジャーナル Mol Cell Neurosci
Abstract The diapause hormone-pheromone biosynthesis activating neuropeptide gene, DH-PBAN, is expressed exclusively in seven pairs of DH-PBAN-producing neurosecretory cells (DHPCs) on the terminally differentiated processes of the subesophageal ganglion (SG). To help reveal the regulatory mechanisms of cell-specific DH-PBAN expression, we identified a cis-regulatory element that regulates expression in DHPCs using the recombinant AcNPV-mediated gene transfer system and a gel-mobility shift assay. Bombyx mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds this element and activates DH-PBAN expression. The BmPitx was expressed in various tissues, including DHPCs in the SG. Suppression of DH-PBAN expression by silencing of the BmPitx successfully induced non-diapaused eggs from a diapause egg producer. To the best of our knowledge, this report is the first to identify a neuropeptide-encoding gene as a target of the Pitx transcriptional regulator in invertebrates. Thus, it is tempting to speculate that functional conservation of Pitx family members on neuropeptide gene expression occurs through a "combinational code mechanism" in both vertebrate and invertebrate in neuroendocrine systems.
巻・号 34(2)
ページ 209-18
公開日 2007-2-1
DOI 10.1016/j.mcn.2006.10.015
PII S1044-7431(06)00239-9
PMID 17166739
MeSH Animals Bombyx / genetics* Cloning, Molecular Gene Expression Regulation / physiology* Genes, Homeobox / physiology* Green Fluorescent Proteins / metabolism Insect Proteins / metabolism* Larva Molecular Sequence Data Neuropeptides / metabolism* Protein Precursors / metabolism* RNA Interference / physiology Reverse Transcriptase Polymerase Chain Reaction / methods
IF 3.182
引用数 16
WOS 分野 NEUROSCIENCES
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