RRC ID 35121
Author Komiya R, Ohyanagi H, Niihama M, Watanabe T, Nakano M, Kurata N, Nonomura K.
Title Rice germline-specific Argonaute MEL1 protein binds to phasiRNAs generated from more than 700 lincRNAs.
Journal Plant J
Abstract Small RNAs that interact with Argonaute (AGO) proteins play central roles in RNA-mediated silencing. MEIOSIS ARRESTED AT LEPTOTENE1 (MEL1), a rice AGO, has specific functions in the development of pre-meiotic germ cells and the progression of meiosis. Here, we show that MEL1, which is located mostly in the cytoplasm of germ cells, associates preferentially with 21-nucleotide phased small interfering RNAs (phasiRNAs) that bear a 5'-terminal cytosine. Most phasiRNAs are derived from 1171 intergenic clusters distributed on all rice chromosomes. From these clusters, over 700 large intergenic, non-coding RNAs (lincRNAs) that contain the consensus sequence complementary to miR2118 are transcribed specifically in inflorescences, and cleaved within the miR2118 site. Cleaved lincRNAs are processed via DICER-LIKE4 (DCL4) protein, resulting in production of phasiRNAs. This study provides the evidence that the miR2118-dependent and the DCL4-dependent pathways are both required for biogenesis of 21-nt phasiRNAs associated with germline-specific MEL1 AGO in rice, and over 700 lincRNAs are key factors for induction of this biogenesis during reproductive-specific stages.
Volume 78(3)
Pages 385-97
Published 2014-5-1
DOI 10.1111/tpj.12483
PMID 24635777
MeSH Argonaute Proteins / genetics Argonaute Proteins / metabolism* Chromosomes, Plant Gene Expression Regulation, Plant Meiosis Oryza / genetics* Plant Proteins / genetics Plant Proteins / metabolism* RNA, Long Noncoding* RNA, Plant / genetics RNA, Plant / metabolism RNA, Small Interfering / metabolism*
IF 5.726
Times Cited 92
WOS Category PLANT SCIENCES
Resource
Rice Sterile strain (STER-NIG0009) (MEL1)