論文 - 詳細
| RRC ID | 35126 |
|---|---|
| 著者 | Natesuntorn W, Iwami K, Matsubara Y, Sasano Y, Sugiyama M, Kaneko Y, Harashima S. |
| タイトル | Genome-wide construction of a series of designed segmental aneuploids in Saccharomyces cerevisiae. |
| ジャーナル | Sci Rep |
| Abstract |
Segmental aneuploidy can play an important role in environmental adaptation. However, study of segmental aneuploids is severely hampered by the difficulty of creating them in a designed fashion. Here, we describe a PCR-mediated chromosome duplication (PCDup) technology that enables the generation of segmental aneuploidy at any desired chromosomal region in Saccharomyces cerevisiae. We constructed multiple strains harboring 100 kb to 200 kb segmental duplications covering the whole of the S. cerevisiae genome. Interestingly, some segmental aneuploidies confer stress tolerance, such as to high temperature, ethanol and strong acids, while others induce cell lethality and stress sensitivity, presumably as result of the simultaneous increases in dosages of multiple genes. We suggest that our PCDup technology will accelerate studies into the phenotypic changes resulting from alteration of gene dosage balance of multiple genes and will provide new insights into the adaptive molecular mechanisms in the genome in segmental aneuploidy-derived human diseases. |
| 巻・号 | 5 |
| ページ | 12510 |
| 公開日 | 2015-7-30 |
| DOI | 10.1038/srep12510 |
| PII | srep12510 |
| PMID | 26224198 |
| PMC | PMC4519793 |
| MeSH | Acids / toxicity Aneuploidy* Chromosome Duplication Chromosomes / genetics* Ethanol / toxicity Gene Dosage Genome, Fungal* Karyotyping Phenotype Plasmids / genetics Plasmids / metabolism Polymerase Chain Reaction Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / genetics* Temperature |
| IF | 3.998 |
| 引用数 | 12 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY4742 |