RRC ID 35134
Author Khatun F, Kurata K, Chuwattanakul V, Sugiyama M, Kaneko Y, Harashima S.
Title Increased transcription of RPL40A and RPL40B is important for the improvement of RNA production in Saccharomyces cerevisiae.
Journal J. Biosci. Bioeng.
Abstract Yeast (Saccharomyces cerevisiae) RNA is an important source of 5'-ribonucleotides that is used in both the food and pharmaceutical industries. Efficient transcription of rDNA is very important to construct yeast strains with high RNA content. The gene RRN10, which encodes, a component of the upstream activation factor, is essential to promote high-level transcription of rDNA. In our previous study, we isolated SupE strain as a dominant suppressor, which showed the ability to restore the severe growth defects and reduced RNA content caused by disruption of the RRN10 gene. SupE strain has multiple mutations which we designated collectively as SUPE. Further analysis on SUPE mutation indicated that RPL40A was responsible for suppression of defect caused by rrn10 disruption. However, there were no base changes in this gene as compared with the parental Δrrn10 strain, thus suggesting that an additional copy of RPL40A suppress the defects caused by Δrrn10 disruption, and that, in SupE strain, these defects are suppressed by increased transcription of RPL40A whose copy is doubled. When multiple copies of RPL40A were combined with SUPE mutation on an RRN10⁺ background, the resultant SupE strain had significantly higher RNA content than wild-type strain. In addition, increased transcription of RPL40B also showed significant effect to restore the growth defect and reduced RNA content caused by Δrrn10 disruption. We propose a model to explain how SUPE mutation increases the transcription of ribosomal protein genes such as RPL40A and RPL40B in SupE strain, resulting in an increase in RNA content.
Volume 116(4)
Pages 423-32
Published 2013-10
DOI 10.1016/j.jbiosc.2013.04.006
PII S1389-1723(13)00127-8
PMID 23669742
MeSH DNA, Ribosomal / genetics DNA-Binding Proteins / genetics Gene Deletion Gene Expression Regulation, Fungal* Mutation / genetics Plasmids / genetics RNA, Fungal / analysis RNA, Fungal / biosynthesis* RNA, Fungal / genetics Ribosomal Proteins / deficiency Ribosomal Proteins / genetics* Ribosomal Proteins / metabolism Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics* Saccharomyces cerevisiae Proteins / metabolism Suppression, Genetic / genetics Transcription Factors / genetics Transcription, Genetic*
IF 2.015
Times Cited 1
Yeast BYP560(pRS314) p617 p1498