| RRC ID |
35142
|
| 著者 |
Suzuki T, Sugiyama M, Wakazono K, Kaneko Y, Harashima S.
|
| タイトル |
Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
To gain more insight into adaptation response to lactic-acid stress in yeast, a genome-wide screening for genes whose disruption caused hypersensitivity to 4.0% l-lactic acid (pH 2.8) was performed using the gene deletion collection of Saccharomyces cerevisiae. We identified 107 genes that contributed significantly to the ability of yeast cells to adapt lactic-acid stress. More than 30% of the genes identified in this screening were newly identified to be involved in mechanisms for adaptation response to lactic acid. We found that protein urmylation by Uba4 and N-terminal acetylation by Nat3 were involved in lactic acid adaptation mechanisms. Functional categorization of the genes followed by microscopic analysis revealed that a variety of cellular functions were involved in adaptation response to lactic acid and function associated with vacuolar transport played important roles in adaptation response to lactic acid. We also found that vacuole fragmented immediately upon exposure to lactic- and hydrochloric-acid stress. In addition, our analysis revealed that lactic-acid stress significantly reduced the amount of intracellular amino acids. Amino acid supplementation recovered the adaptation deficiency to lactic acid, suggesting that intracellular amino-acid homeostasis plays important roles in adaptation response to lactic-acid stress. These data suggest that enhancing vacuolar integrity, as well as maintaining intracellular amino-acid homeostasis may be an efficient approach to confer resistance to lactic-acid stress.
|
| 巻・号 |
113(4)
|
| ページ |
421-30
|
| 公開日 |
2012-4-1
|
| DOI |
10.1016/j.jbiosc.2011.11.010
|
| PII |
S1389-1723(11)00457-9
|
| PMID |
22177309
|
| MeSH |
Adaptation, Physiological / genetics
Amino Acids / metabolism
Amino Acids / pharmacology
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation, Fungal
Genome, Fungal / genetics
Homeostasis / genetics
Lactic Acid / pharmacology
Saccharomyces cerevisiae / drug effects
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism
Stress, Physiological / genetics
Vacuoles / drug effects
Vacuoles / metabolism*
|
| IF |
2.366
|
| 引用数 |
12
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 酵母 |
NA |
