論文 - 詳細
| RRC ID | 35177 |
|---|---|
| 著者 | Yamashita A, Morioka M, Yahara Y, Okada M, Kobayashi T, Kuriyama S, Matsuda S, Tsumaki N. |
| タイトル | Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs. |
| ジャーナル | Stem Cell Reports |
| Abstract |
Defects in articular cartilage ultimately result in loss of joint function. Repairing cartilage defects requires cell sources. We developed an approach to generate scaffoldless hyaline cartilage from human induced pluripotent stem cells (hiPSCs). We initially generated an hiPSC line that specifically expressed GFP in cartilage when teratoma was formed. We optimized the culture conditions and found BMP2, transforming growth factor β1 (TGF-β1), and GDF5 critical for GFP expression and thus chondrogenic differentiation of the hiPSCs. The subsequent use of scaffoldless suspension culture contributed to purification, producing homogenous cartilaginous particles. Subcutaneous transplantation of the hiPSC-derived particles generated hyaline cartilage that expressed type II collagen, but not type I collagen, in immunodeficiency mice. Transplantation of the particles into joint surface defects in immunodeficiency rats and immunosuppressed mini-pigs indicated that neocartilage survived and had potential for integration into native cartilage. The immunodeficiency mice and rats suffered from neither tumors nor ectopic tissue formation. The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects. |
| 巻・号 | 4(3) |
| ページ | 404-18 |
| 公開日 | 2015-3-10 |
| DOI | 10.1016/j.stemcr.2015.01.016 |
| PII | S2213-6711(15)00037-5 |
| PMID | 25733017 |
| PMC | PMC4375934 |
| MeSH | Animals Biomarkers Cell Culture Techniques Cell Differentiation* Cell Line Cell Transformation, Neoplastic Chondrocytes / cytology Chondrogenesis Gene Expression Gene Knock-In Techniques Genes, Reporter Humans Hyaline Cartilage / cytology* Hyaline Cartilage / metabolism* Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism* Mice Mice, SCID Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Stem Cell Transplantation Swine Swine, Miniature Transgenes |
| IF | 6.032 |
| 引用数 | 81 |
| WOS 分野 | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 49 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | F344-Il2rgem2Kyo (strainID=979) |