| RRC ID |
35203
|
| Author |
Das G, Thotala DK, Kapoor S, Karunanithi S, Thakur SS, Singh NS, Varshney U.
|
| Title |
Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli.
|
| Journal |
EMBO J
|
| Abstract |
Translation initiation from the ribosomal P-site is the specialty of the initiator tRNAs (tRNA(fMet)). Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. How this feature is exploited by ribosomes has remained unclear. Using a genetic screen, we have isolated an Escherichia coli strain, carrying a G122D mutation in folD, which allows initiation with the tRNA(fMet) containing mutations in one, two or all the three G-C base pairs. The strain shows a severe deficiency of methionine and S-adenosylmethionine, and lacks nucleoside methylations in rRNA. Targeted mutations in the methyltransferase genes have revealed a connection between the rRNA modifications and the fundamental process of the initiator tRNA selection by the ribosome.
|
| Volume |
27(6)
|
| Pages |
840-51
|
| Published |
2008-3-19
|
| DOI |
10.1038/emboj.2008.20
|
| PII |
emboj200820
|
| PMID |
18288206
|
| PMC |
PMC2274930
|
| MeSH |
5,10-Methylenetetrahydrofolate Reductase (FADH2) / biosynthesis
5,10-Methylenetetrahydrofolate Reductase (FADH2) / genetics
Amino Acid Substitution / genetics
Base Sequence
Escherichia coli Proteins / biosynthesis
Escherichia coli Proteins / genetics*
Methylation
Molecular Sequence Data
Mutation
Peptide Chain Initiation, Translational / genetics*
RNA, Bacterial / metabolism
RNA, Ribosomal, 16S / metabolism*
RNA, Ribosomal, 16S / physiology
RNA, Transfer, Met / genetics
Ribosomes / metabolism*
|
| IF |
9.889
|
| Times Cited |
46
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
|
