論文 - 詳細
| RRC ID | 35204 |
|---|---|
| 著者 | Ebersbach G, Galli E, Møller-Jensen J, Löwe J, Gerdes K. |
| タイトル | Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division. |
| ジャーナル | Mol Microbiol |
| Abstract |
Formation of the Z ring is the first known event in bacterial cell division. However, it is not yet known how the assembly and contraction of the Z ring are regulated. Here, we identify a novel cell division factor ZapB in Escherichia coli that simultaneously stimulates Z ring assembly and cell division. Deletion of zapB resulted in delayed cell division and the formation of ectopic Z rings and spirals, whereas overexpression of ZapB resulted in nucleoid condensation and aberrant cell divisions. Localization of ZapB to the divisome depended on FtsZ but not FtsA, ZipA or FtsI, and ZapB interacted with FtsZ in a bacterial two-hybrid analysis. The simultaneous inactivation of FtsA and ZipA prevented Z ring assembly and ZapB localization. Time lapse microscopy showed that ZapB-GFP is present at mid-cell in a pattern very similar to that of FtsZ. Cells carrying a zapB deletion and the ftsZ84(ts) allele exhibited a synthetic sick phenotype and aberrant cell divisions. The crystal structure showed that ZapB exists as a dimer that is 100% coiled-coil. In vitro, ZapB self-assembled into long filaments and bundles. These results raise the possibility that ZapB stimulates Z ring formation directly via its capacity to self-assemble into larger structures. |
| 巻・号 | 68(3) |
| ページ | 720-35 |
| 公開日 | 2008-5-1 |
| DOI | 10.1111/j.1365-2958.2008.06190.x |
| PII | MMI6190 |
| PMID | 18394147 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Carrier Proteins / metabolism Cell Cycle Proteins / chemistry* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Division* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism Escherichia coli / cytology* Escherichia coli / metabolism Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Fluorescence Models, Molecular Penicillin-Binding Proteins / metabolism Peptidoglycan Glycosyltransferase / metabolism Protein Binding Protein Structure, Tertiary Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Deletion Two-Hybrid System Techniques |
| IF | 3.418 |
| 引用数 | 85 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | |