RRC ID 35214
著者 Lambert MA, Smith SG.
タイトル The PagN protein of Salmonella enterica serovar Typhimurium is an adhesin and invasin.
ジャーナル BMC Microbiol
Abstract BACKGROUND:The pagN gene of Salmonella enterica serovar Typhimurium is a PhoP-regulated gene that is up-regulated during growth within macrophages and in vivo in murine models of infection. The PagN protein displays similarity to the Hek and Tia invasins/adhesins of Escherichia coli. Thus far no function has been ascribed to the PagN protein.
RESULTS:Here we show that the outer membrane located PagN protein mediates agglutination of red blood cells and that this can be masked by LPS. When expressed in Escherichia coli the PagN protein supports adhesion to and invasion of mammalian cells in a manner that is dependent on cytoskeletal rearrangements. S. enterica sv Typhimurium pagN mutants display a reduction in adhesion to and invasion of epithelial cells. Finally, we demonstrate that over-expression of PagN in a SPI-1 mutant can partially compensate for the lack of a functional invasasome.
CONCLUSION:PagN is an outer membrane protein that may contribute to the virulence of S. Typhimurium. This protein is a haemagglutinin and contributes to the adherence to mammalian cells. In addition, PagN can mediate high-level invasion of CHO-K1 cells. Previously,pagN mutants have been shown to be less competitive in vivo and thus this may be due to their lessened ability to interact with mammalian cells. Finally PagN can be added to an ever-growing repertoire of factors that contribute to the pathogenesis of Salmonella.
巻・号 8
ページ 142
公開日 2008-9-8
DOI 10.1186/1471-2180-8-142
PII 1471-2180-8-142
PMID 18778463
PMC PMC2553418
MeSH Adhesins, Bacterial / genetics Adhesins, Bacterial / metabolism* Animals CHO Cells Cricetinae Cricetulus HT29 Cells Hemagglutinins / metabolism Humans Mutation Proteins / metabolism Salmonella typhimurium / genetics Salmonella typhimurium / metabolism* Salmonella typhimurium / pathogenicity
IF 2.989
引用数 41
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) pACYC184? pBR322?