Reference - Detail
| RRC ID | 35225 |
|---|---|
| Author | Krieg S, Huché F, Diederichs K, Izadi-Pruneyre N, Lecroisey A, Wandersman C, Delepelaire P, Welte W. |
| Title | Heme uptake across the outer membrane as revealed by crystal structures of the receptor-hemophore complex. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Gram-negative bacteria use specific heme uptake systems, relying on outer membrane receptors and excreted heme-binding proteins (hemophores) to scavenge and actively transport heme. To unravel the unknown molecular details involved, we present 3 structures of the Serratia marcescens receptor HasR in complex with its hemophore HasA. The transfer of heme over a distance of 9 A from its high-affinity site in HasA into a site of lower affinity in HasR is coupled with the exergonic complex formation of the 2 proteins. Upon docking to the receptor, 1 of the 2 axial heme coordinations of the hemophore is initially broken, but the position and orientation of the heme is preserved. Subsequently, steric displacement of heme by a receptor residue ruptures the other axial coordination, leading to heme transfer into the receptor. |
| Volume | 106(4) |
| Pages | 1045-50 |
| Published | 2009-1-27 |
| DOI | 10.1073/pnas.0809406106 |
| PII | 0809406106 |
| PMID | 19144921 |
| PMC | PMC2633585 |
| MeSH | Apoproteins / chemistry Apoproteins / metabolism Bacterial Proteins / chemistry* Bacterial Proteins / metabolism Biological Transport Calorimetry Carrier Proteins / chemistry* Carrier Proteins / metabolism Cell Membrane / metabolism* Crystallography, X-Ray Heme / chemistry Heme / metabolism* Heme-Binding Proteins Hemeproteins / chemistry* Hemeproteins / metabolism Ligands Membrane Proteins / chemistry* Membrane Proteins / metabolism Models, Molecular Protein Structure, Secondary Receptors, Cell Surface / chemistry* Receptors, Cell Surface / metabolism Serratia marcescens / chemistry* Surface Properties |
| IF | 9.412 |
| Times Cited | 110 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |