RRC ID 35227
著者 Liu YS, Zhang YQ, Yang LX, Yao TJ, Xiao CL.
タイトル Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa.
ジャーナル Biotechnol Lett
Abstract To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in prokaryotes.
巻・号 31(10)
ページ 1571-5
公開日 2009-10-1
DOI 10.1007/s10529-009-0046-x
PMID 19543856
MeSH Anti-Bacterial Agents / pharmacology Bacterial Outer Membrane Proteins / antagonists & inhibitors Gene Knockdown Techniques / methods* Gene Silencing* Membrane Transport Proteins Microbial Sensitivity Tests Pseudomonas aeruginosa / genetics* RNA, Complementary / genetics*
IF 1.977
引用数 5
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
原核生物(大腸菌)