RRC ID 35235
Author Shimada T, Yamamoto K, Ishihama A.
Title Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux.
Journal J Bacteriol
Abstract LeuO, a LysR family transcription factor, exists in a wide variety of bacteria of the family Enterobacteriaceae and is involved in the regulation of as yet unidentified genes affecting the stress response and pathogenesis expression. Using genomic screening by systematic evolution of ligands by exponential enrichment (SELEX) in vitro, a total of 106 DNA sequences were isolated from 12 different regions of the Escherichia coli genome. All of the SELEX fragments formed complexes in vitro with purified LeuO. After Northern blot analysis of the putative target genes located downstream of the respective LeuO-binding sequence, a total of nine genes were found to be activated by LeuO, while three genes were repressed by LeuO. The LeuO target gene collection included several multidrug resistance genes. A phenotype microarray assay was conducted to identify the gene(s) responsible for drug resistance and the drug species that are under the control of the LeuO target gene(s). The results described herein indicate that the yjcRQP operon, one of the LeuO targets, is involved in sensitivity control against sulfa drugs. We propose to rename the yjcRQP genes the sdsRQP genes (sulfa drug sensitivity determinant).
Volume 191(14)
Pages 4562-71
Published 2009-7-1
DOI 10.1128/JB.00108-09
PII JB.00108-09
PMID 19429622
PMC PMC2704711
MeSH Anti-Infective Agents / metabolism* Biological Transport Drug Resistance, Bacterial* Escherichia coli / drug effects* Escherichia coli / physiology* Escherichia coli Proteins / physiology* Gene Expression Profiling Gene Expression Regulation, Bacterial* Membrane Transport Proteins / metabolism SELEX Aptamer Technique Sulfanilamides / metabolism* Transcription Factors / physiology*
IF 3.006
Times Cited 41
Prokaryotes E. coli ME9062(BW25113)(Keio) JW0075-KC JW1225-KC JW3233-KC JW2730-KC JW4043-KC JW0451-KC(acrB)?