RRC ID |
35243
|
著者 |
Tarry M, Arends SJ, Roversi P, Piette E, Sargent F, Berks BC, Weiss DS, Lea SM.
|
タイトル |
The Escherichia coli cell division protein and model Tat substrate SufI (FtsP) localizes to the septal ring and has a multicopper oxidase-like structure.
|
ジャーナル |
J Mol Biol
|
Abstract |
The Escherichia coli protein SufI (FtsP) has recently been proposed to be a component of the cell division apparatus. The SufI protein is also in widespread experimental use as a model substrate in studies of the Tat (twin arginine translocation) protein transport system. We have used SufI-GFP (green fluorescent protein) fusions to show that SufI localizes to the septal ring in the dividing cell. We have also determined the structure of SufI by X-ray crystallography to a resolution of 1.9 A. SufI is structurally related to the multicopper oxidase superfamily but lacks metal cofactors. The structure of SufI suggests it serves a scaffolding rather than an enzymatic role in the septal ring and reveals regions of the protein likely to be involved in the protein-protein interactions required to assemble SufI at the septal ring.
|
巻・号 |
386(2)
|
ページ |
504-19
|
公開日 |
2009-2-20
|
DOI |
10.1016/j.jmb.2008.12.043
|
PII |
S0022-2836(08)01564-7
|
PMID |
19135451
|
PMC |
PMC2661564
|
MeSH |
Artificial Gene Fusion
Cell Division*
Crystallography, X-Ray
Escherichia coli / chemistry*
Escherichia coli / physiology*
Escherichia coli Proteins / chemistry*
Escherichia coli Proteins / metabolism*
Genes, Reporter
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Models, Molecular
Protein Structure, Tertiary
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
|
IF |
4.76
|
引用数 |
34
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
Keio collection? |