論文 - 詳細
| RRC ID | 35243 |
|---|---|
| 著者 | Tarry M, Arends SJ, Roversi P, Piette E, Sargent F, Berks BC, Weiss DS, Lea SM. |
| タイトル | The Escherichia coli cell division protein and model Tat substrate SufI (FtsP) localizes to the septal ring and has a multicopper oxidase-like structure. |
| ジャーナル | J Mol Biol |
| Abstract |
The Escherichia coli protein SufI (FtsP) has recently been proposed to be a component of the cell division apparatus. The SufI protein is also in widespread experimental use as a model substrate in studies of the Tat (twin arginine translocation) protein transport system. We have used SufI-GFP (green fluorescent protein) fusions to show that SufI localizes to the septal ring in the dividing cell. We have also determined the structure of SufI by X-ray crystallography to a resolution of 1.9 A. SufI is structurally related to the multicopper oxidase superfamily but lacks metal cofactors. The structure of SufI suggests it serves a scaffolding rather than an enzymatic role in the septal ring and reveals regions of the protein likely to be involved in the protein-protein interactions required to assemble SufI at the septal ring. |
| 巻・号 | 386(2) |
| ページ | 504-19 |
| 公開日 | 2009-2-20 |
| DOI | 10.1016/j.jmb.2008.12.043 |
| PII | S0022-2836(08)01564-7 |
| PMID | 19135451 |
| PMC | PMC2661564 |
| MeSH | Artificial Gene Fusion Cell Division* Crystallography, X-Ray Escherichia coli / chemistry* Escherichia coli / physiology* Escherichia coli Proteins / chemistry* Escherichia coli Proteins / metabolism* Genes, Reporter Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Models, Molecular Protein Structure, Tertiary Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism |
| IF | 4.76 |
| 引用数 | 34 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection? |