論文 - 詳細
| RRC ID | 35245 |
|---|---|
| 著者 | Fagan RP, Lambert MA, Smith SG. |
| タイトル | The hek outer membrane protein of Escherichia coli strain RS218 binds to proteoglycan and utilizes a single extracellular loop for adherence, invasion, and autoaggregation. |
| ジャーナル | Infect Immun |
| Abstract |
Escherichia coli is the principal gram-negative causative agent of sepsis and meningitis in neonates. The pathogenesis of meningitis due to E. coli K1 involves mucosal colonization, transcytosis of epithelial cells, survival in the bloodstream, and eventually invasion of the meninges. The last two aspects have been well characterized at a molecular level. Less is known about the early stages of pathogenesis, i.e., adhesion to and invasion of epithelial cells. We have previously reported that the Hek protein causes autoaggregation and can mediate adherence to and invasion of epithelial cells. Here, we report that Hek-mediated adherence is dependent on binding to glycosoaminoglycan, in particular, heparin. The ability to hemagglutinate, autoaggregate, adhere, and invade is contingent on a putative 25-amino-acid loop that is exposed to the outside of the bacterial cells. |
| 巻・号 | 76(3) |
| ページ | 1135-42 |
| 公開日 | 2008-3-1 |
| DOI | 10.1128/IAI.01327-07 |
| PII | IAI.01327-07 |
| PMID | 18160475 |
| PMC | PMC2258800 |
| MeSH | Adhesins, Escherichia coli / chemistry Adhesins, Escherichia coli / metabolism* Animals Bacterial Adhesion* Cell Line Chondroitin Sulfates / metabolism Cricetinae Dextran Sulfate / metabolism Epithelial Cells / microbiology* Escherichia coli / physiology* Heparin / metabolism* Heparitin Sulfate / metabolism Models, Molecular Protein Binding Protein Interaction Mapping Protein Structure, Tertiary Sequence Deletion |
| IF | 3.201 |
| 引用数 | 23 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | |