論文 - 詳細
| RRC ID | 35248 |
|---|---|
| 著者 | Yamamoto K, Matsumoto F, Minagawa S, Oshima T, Fujita N, Ogasawara N, Ishihama A. |
| タイトル | Characterization of CitA-CitB signal transduction activating genes involved in anaerobic citrate catabolism in Escherichia coli. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
In Escherichia coli, CitA is a membrane-associated sensor histidine kinase that phosphorylates CitB, the response regulator. It is predicated to play a key role in anaerobic citrate catabolism. The citrate-binding site in CitA is located within its periplasmic domain, while the cytoplasmic domain (CitA-C) is involved in autophosphorylation. We found that autophosphorylation in vitro of CitA-C was induced by DTT. Using the whole set of CitA-C derivatives containing Cys-Ala substitution(s), Cys at 529 was found to be essential to the redox-sensing of autophosphorylation. The phosphorylated CitA-C transferred a phosphate to CitB. DNase-I footprinting assay indicated that CitB specifically bound on the intergenic region between the citA and citC genes. These results characterize the molecular mechanism of the CitA-CitB signal transduction system in E. coli. |
| 巻・号 | 73(2) |
| ページ | 346-50 |
| 公開日 | 2009-2-1 |
| DOI | 10.1271/bbb.80586 |
| PII | JST.JSTAGE/bbb/80586 |
| PMID | 19202292 |
| MeSH | Anaerobiosis Citric Acid / metabolism* Cytoplasm / metabolism Escherichia coli / cytology Escherichia coli / genetics* Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* Genes, Bacterial* Oxidation-Reduction Phosphorylation Protein Kinases / chemistry Protein Kinases / metabolism* Protein Structure, Tertiary Signal Transduction / genetics* Substrate Specificity Transcription Factors / metabolism* |
| IF | 1.516 |
| 引用数 | 9 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | |