| RRC ID |
35248
|
| 著者 |
Yamamoto K, Matsumoto F, Minagawa S, Oshima T, Fujita N, Ogasawara N, Ishihama A.
|
| タイトル |
Characterization of CitA-CitB signal transduction activating genes involved in anaerobic citrate catabolism in Escherichia coli.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
In Escherichia coli, CitA is a membrane-associated sensor histidine kinase that phosphorylates CitB, the response regulator. It is predicated to play a key role in anaerobic citrate catabolism. The citrate-binding site in CitA is located within its periplasmic domain, while the cytoplasmic domain (CitA-C) is involved in autophosphorylation. We found that autophosphorylation in vitro of CitA-C was induced by DTT. Using the whole set of CitA-C derivatives containing Cys-Ala substitution(s), Cys at 529 was found to be essential to the redox-sensing of autophosphorylation. The phosphorylated CitA-C transferred a phosphate to CitB. DNase-I footprinting assay indicated that CitB specifically bound on the intergenic region between the citA and citC genes. These results characterize the molecular mechanism of the CitA-CitB signal transduction system in E. coli.
|
| 巻・号 |
73(2)
|
| ページ |
346-50
|
| 公開日 |
2009-2-1
|
| DOI |
10.1271/bbb.80586
|
| PII |
JST.JSTAGE/bbb/80586
|
| PMID |
19202292
|
| MeSH |
Anaerobiosis
Citric Acid / metabolism*
Cytoplasm / metabolism
Escherichia coli / cytology
Escherichia coli / genetics*
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / metabolism*
Genes, Bacterial*
Oxidation-Reduction
Phosphorylation
Protein Kinases / chemistry
Protein Kinases / metabolism*
Protein Structure, Tertiary
Signal Transduction / genetics*
Substrate Specificity
Transcription Factors / metabolism*
|
| IF |
1.516
|
| 引用数 |
9
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
|
