RRC ID 35250
Author Acuña LG, Calderón IL, Elías AO, Castro ME, Vásquez CC.
Title Expression of the yggE gene protects Escherichia coli from potassium tellurite-generated oxidative stress.
Journal Arch Microbiol
Abstract Potassium tellurite is highly toxic to most forms of life and specific bacterial tellurite defense mechanisms are not fully understood to date. Recent evidence suggests that tellurite would exert its toxic effects, at least in part, through the generation of superoxide anion that occurs concomitantly with intracellular tellurite (Te(4+)) reduction to elemental tellurium (Te(o)). In this work the putative antioxidant role of YggE from Escherichia coli, a highly conserved protein in several bacterial species and whose function is still a matter of speculation, was studied. When exposed to tellurite, E. coli lacking yggE exhibited increased activity of superoxide dismutase and fumarase C, augmented levels of reactive oxygen species and high concentration of carbonyl groups in proteins. Upon genetic complementation with the homologous yggE gene these values were restored to those observed in the parental, isogenic, wild type strain, suggesting a direct participation of YggE in E. coli tolerance to tellurite.
Volume 191(5)
Pages 473-6
Published 2009-5
DOI 10.1007/s00203-009-0473-z
PMID 19330318
MeSH Escherichia coli / drug effects* Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Escherichia coli Proteins / physiology* Fumarate Hydratase / metabolism Gene Deletion Genetic Complementation Test Oxidative Stress* Protein Carbonylation Reactive Oxygen Species / metabolism Superoxide Dismutase / metabolism Tellurium / toxicity*
IF 1.642
Times Cited 17
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli JW2889-KC(yggE)?