RRC ID 35266
著者 Gore AL, Payne SM.
タイトル CsrA and Cra influence Shigella flexneri pathogenesis.
ジャーナル Infect Immun
Abstract Shigella flexneri is a facultative intracellular pathogen that invades and disrupts the colonic epithelium. In order to thrive in the host, S. flexneri must adapt to environmental conditions in the gut and within the eukaryotic cytosol, including variability in the available carbon sources and other nutrients. We examined the roles of the carbon consumption regulators CsrA and Cra in a cell culture model of S. flexneri virulence. CsrA is an activator of glycolysis and a repressor of gluconeogenesis, and a csrA mutant had decreased attachment and invasion of cultured cells. Conversely, Cra represses glycolysis and activates gluconeogenesis, and the cra mutant had an increase in both attachment and invasion compared to the wild-type strain. Both mutants were defective in plaque formation. The importance of the glycolytic pathway in invasion and plaque formation was confirmed by testing the effect of a mutation in the glycolysis gene pfkA. The pfkA mutant was noninvasive and had cell surface alterations as indicated by decreased sensitivity to SDS and an altered lipopolysaccharide profile. The loss of invasion by the csrA and pfkA mutants was due to decreased expression of the S. flexneri virulence factor regulators virF and virB, resulting in decreased production of Shigella invasion plasmid antigens (Ipa). These data indicate that regulation of carbon metabolism and expression of the glycolysis gene pfkA are critical for synthesis of the virulence gene regulators VirF and VirB, and both the glycolytic and gluconeogenic pathways influence steps in S. flexneri invasion and plaque formation.
巻・号 78(11)
ページ 4674-82
公開日 2010-11-1
DOI 10.1128/IAI.00589-10
PII IAI.00589-10
PMID 20713625
PMC PMC2976347
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cells, Cultured Epithelial Cells / microbiology Gene Expression Regulation, Bacterial* Genes, Regulator Gluconeogenesis / drug effects Glycolysis / drug effects Humans Intestines / cytology Intestines / microbiology Phosphofructokinase-1 / genetics Phosphofructokinase-1 / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Shigella flexneri / genetics Shigella flexneri / metabolism Shigella flexneri / pathogenicity* Virulence Virulence Factors / genetics Virulence Factors / metabolism*
IF 3.201
引用数 43
WOS 分野 INFECTIOUS DISEASES IMMUNOLOGY
リソース情報
原核生物(大腸菌) JW0078-KC JW1899-KC JW2757-KC JW3221-KC JW3887-KC