| RRC ID |
35269
|
| 著者 |
Hoshino Y, Fujii S, Shinonaga H, Arai K, Saito F, Fukai T, Satoh H, Miyazaki Y, Ishikawa J.
|
| タイトル |
Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.
|
| ジャーナル |
J Antibiot (Tokyo)
|
| Abstract |
We demonstrated that the rox gene of Nocardia farcinica encodes a rifampicin monooxygenase capable of converting rifampicin to a new compound 2'-N-hydroxy-4-oxo-rifampicin with a markedly lowered antibiotic activity. The deletion mutation (Deltarox) of the rox gene gave no significant influence to the rifampicin resistance of N. farcinica. However, transformation with a plasmid containing an overexpressing the rox gene markedly raised the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. On the other hand, rifampicin was decolorized by the wild-type strain, whereas it remained intact when incubated with the Deltarox strain. Based on these results, it will be conclusive that the rox gene is capable of initiating rifampicin degradation with a new metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in N. farcinica.
|
| 巻・号 |
63(1)
|
| ページ |
23-8
|
| 公開日 |
2010-1-1
|
| DOI |
10.1038/ja.2009.116
|
| PII |
ja2009116
|
| PMID |
19942945
|
| MeSH |
Anti-Bacterial Agents / metabolism*
Anti-Bacterial Agents / pharmacology
Bacteria / drug effects
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Biotransformation
Drug Resistance, Bacterial*
Gene Deletion
Gene Dosage
Gene Order
Magnetic Resonance Spectroscopy
Microbial Sensitivity Tests
Mixed Function Oxygenases / genetics
Mixed Function Oxygenases / metabolism*
Molecular Structure
Nocardia / drug effects
Nocardia / enzymology*
Nocardia / genetics
Plasmids
Rifampin / metabolism*
Rifampin / pharmacology
|
| IF |
2.668
|
| 引用数 |
24
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
IMMUNOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
pK18mobsacB |
