論文 - 詳細
| RRC ID | 35269 |
|---|---|
| 著者 | Hoshino Y, Fujii S, Shinonaga H, Arai K, Saito F, Fukai T, Satoh H, Miyazaki Y, Ishikawa J. |
| タイトル | Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance. |
| ジャーナル | J Antibiot (Tokyo) |
| Abstract |
We demonstrated that the rox gene of Nocardia farcinica encodes a rifampicin monooxygenase capable of converting rifampicin to a new compound 2'-N-hydroxy-4-oxo-rifampicin with a markedly lowered antibiotic activity. The deletion mutation (Deltarox) of the rox gene gave no significant influence to the rifampicin resistance of N. farcinica. However, transformation with a plasmid containing an overexpressing the rox gene markedly raised the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. On the other hand, rifampicin was decolorized by the wild-type strain, whereas it remained intact when incubated with the Deltarox strain. Based on these results, it will be conclusive that the rox gene is capable of initiating rifampicin degradation with a new metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in N. farcinica. |
| 巻・号 | 63(1) |
| ページ | 23-8 |
| 公開日 | 2010-1-1 |
| DOI | 10.1038/ja.2009.116 |
| PII | ja2009116 |
| PMID | 19942945 |
| MeSH | Anti-Bacterial Agents / metabolism* Anti-Bacterial Agents / pharmacology Bacteria / drug effects Bacterial Proteins / genetics Bacterial Proteins / metabolism* Biotransformation Drug Resistance, Bacterial* Gene Deletion Gene Dosage Gene Order Magnetic Resonance Spectroscopy Microbial Sensitivity Tests Mixed Function Oxygenases / genetics Mixed Function Oxygenases / metabolism* Molecular Structure Nocardia / drug effects Nocardia / enzymology* Nocardia / genetics Plasmids Rifampin / metabolism* Rifampin / pharmacology |
| IF | 2.668 |
| 引用数 | 24 |
| WOS 分野 | PHARMACOLOGY & PHARMACY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | pK18mobsacB |