Reference - Detail
| RRC ID | 35271 |
|---|---|
| Author | Kapoor S, Das G, Varshney U. |
| Title | Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli. |
| Journal | Nucleic Acids Res |
| Abstract |
The accuracy of the initiator tRNA (tRNA(fMet)) selection in the ribosomal P-site is central to the fidelity of protein synthesis. A highly conserved occurrence of three consecutive G-C base pairs in the anticodon stem of tRNA(fMet) contributes to its preferential selection in the P-site. In a genetic screen, using a plasmid borne copy of an inactive tRNA(fMet) mutant wherein the three G-C base pairs were changed, we isolated Escherichia coli strains that allow efficient initiation with the tRNA(fMet) mutant. Here, extensive characterization of two such strains revealed novel mutations in the metZWV promoter severely compromising tRNA(fMet) levels. Low cellular abundance of the chromosomally encoded tRNA(fMet) allows efficient initiation with the tRNA(fMet) mutant and an elongator tRNA(Gln), revealing that a high abundance of the cellular tRNA(fMet) is crucial for the fidelity of initiator tRNA selection on the ribosomal P-site in E. coli. We discuss possible implications of the changes in the cellular tRNA(fMet) abundance in proteome remodeling. |
| Volume | 39(1) |
| Pages | 202-12 |
| Published | 2011-1-1 |
| DOI | 10.1093/nar/gkq760 |
| PII | gkq760 |
| PMID | 20798174 |
| PMC | PMC3017606 |
| MeSH | Anticodon / chemistry Base Pairing Chromosome Mapping Cold Temperature Escherichia coli / genetics* Escherichia coli / growth & development Escherichia coli / metabolism Genetic Loci Mutation Peptide Chain Initiation, Translational* RNA, Transfer, Gln / metabolism RNA, Transfer, Met / chemistry RNA, Transfer, Met / genetics* RNA, Transfer, Met / metabolism Ribosomes / chemistry Ribosomes / metabolism* Suppression, Genetic |
| IF | 11.502 |
| Times Cited | 20 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |