RRC ID 35274
Author Liang FT, Xu Q, Sikdar R, Xiao Y, Cox JS, Doerrler WT.
Title BB0250 of Borrelia burgdorferi is a conserved and essential inner membrane protein required for cell division.
Journal J. Bacteriol.
Abstract The gene bb0250 of Borrelia burgdorferi is a homolog of the dedA family, encoding integral inner membrane proteins that are present in nearly all species of bacteria. To date, no precise function has been attributed to any dedA gene. Unlike many bacterial species, such as Escherichia coli, which has eight dedA genes, B. burgdorferi possesses only one, annotated bb0250, providing a unique opportunity to investigate the functions of the dedA family. Here, we show that bb0250 is able to restore normal growth and cell division to a temperature-sensitive E. coli mutant with simultaneous deletions of two dedA genes, yqjA and yghB, and encodes a protein that localizes to the inner membrane of E. coli. The bb0250 gene could be deleted from B. burgdorferi only after introduction of a promoterless bb0250 under the control of an inducible lac promoter, indicating that it is an essential gene in this organism. Growth of the mutant in the absence of isopropyl-β-d-thiogalactopyranoside resulted in cell death, preceded by cell division defects characterized by elongated cells and membrane bulges, demonstrating that bb0250 is required for proper cell division and envelope integrity. Finally, we show that BB0250 depletion leads to imbalanced membrane phospholipid composition in borrelia. These results demonstrate a strong conservation of function of the dedA gene family across diverse species of Gram-negative bacteria and a requirement for this protein family for normal membrane lipid composition and cell division.
Volume 192(23)
Pages 6105-15
Published 2010-12
DOI 10.1128/JB.00571-10
PII JB.00571-10
PMID 20870761
PMC PMC2981195
MeSH Amino Acid Sequence Bacterial Proteins / genetics Bacterial Proteins / metabolism* Borrelia burgdorferi / cytology Borrelia burgdorferi / genetics Borrelia burgdorferi / physiology* Cell Death Cell Division* Cell Membrane / chemistry Conserved Sequence Escherichia coli / genetics Escherichia coli Proteins Gene Deletion Genes, Essential Genetic Complementation Test Membrane Proteins / deficiency Membrane Proteins / genetics Membrane Proteins / metabolism* Microscopy, Electron, Scanning Molecular Sequence Data Phospholipids / analysis Promoter Regions, Genetic Transcriptional Activation
IF 3.219
Times Cited 13
WOS Category MICROBIOLOGY
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Prokaryotes E. coli ?