| RRC ID |
35284
|
| Author |
Shoji S, Janssen BD, Hayes CS, Fredrick K.
|
| Title |
Translation factor LepA contributes to tellurite resistance in Escherichia coli but plays no apparent role in the fidelity of protein synthesis.
|
| Journal |
Biochimie
|
| Abstract |
LepA is a translational GTPase highly conserved in bacterial lineages. While it has been shown that LepA can catalyze reverse ribosomal translocation in vitro, the role of LepA in the cell remains unclear. Here, we show that deletion of the lepA gene (DeltalepA) in Escherichia coli causes hypersensitivity to potassium tellurite and penicillin G, but has no appreciable effect on growth under many other conditions. DeltalepA does not increase miscoding or frameshifting errors under normal or stress conditions, indicating that LepA does not contribute to the fidelity of translation. Overexpression of LepA interferes with tmRNA-mediated peptide tagging and A-site mRNA cleavage, suggesting that LepA is a bona fide translation factor that can act on stalled ribosomes with a vacant A site in vivo. Together these results lead us to hypothesize that LepA is involved in co-translational folding of proteins that are otherwise vulnerable to tellurite oxidation.
|
| Volume |
92(2)
|
| Pages |
157-63
|
| Published |
2010-2-1
|
| DOI |
10.1016/j.biochi.2009.11.002
|
| PII |
S0300-9084(09)00306-X
|
| PMID |
19925844
|
| PMC |
PMC2815024
|
| MeSH |
Drug Resistance, Bacterial*
Escherichia coli / cytology
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli Proteins / biosynthesis*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
GTP Phosphohydrolases / deficiency
GTP Phosphohydrolases / genetics
Gene Deletion
Oxidants / pharmacology
Peptide Initiation Factors
Phosphoproteins / deficiency
Phosphoproteins / genetics
Protein Biosynthesis / physiology*
RNA, Messenger / genetics
Tellurium / pharmacology*
Transcriptional Elongation Factors / deficiency
Transcriptional Elongation Factors / genetics
Transcriptional Elongation Factors / metabolism*
|
| IF |
3.413
|
| Times Cited |
40
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
ME9062(BW25113)(Keio)
JW2553-KC
JW5771-KC |
