RRC ID 35286
Author Sinha J, Reyes SJ, Gallivan JP.
Title Reprogramming bacteria to seek and destroy an herbicide.
Journal Nat. Chem. Biol.
Abstract A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this riboswitch can reprogram bacteria to migrate in the presence of atrazine. Finally, we show that incorporating a gene from an atrazine catabolic pathway allows these cells to seek and destroy atrazine.
Volume 6(6)
Pages 464-70
Published 2010-6
DOI 10.1038/nchembio.369
PII nchembio.369
PMID 20453864
PMC PMC2873063
MeSH Acetone / metabolism Atrazine / chemical synthesis Atrazine / metabolism* Atrazine / pharmacology Bacteria / drug effects Bacteria / genetics* Bacteria / metabolism* Bacterial Physiological Phenomena Base Sequence Cell Movement Cloning, Molecular DNA, Bacterial / genetics Ethanolamines / metabolism Gene Expression Regulation, Bacterial Herbicides / chemical synthesis Herbicides / metabolism* Kinetics Light Molecular Sequence Data RNA, Bacterial / chemistry RNA, Bacterial / genetics RNA, Bacterial / metabolism beta-Galactosidase / metabolism
IF 13.843
Times Cited 106
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes E. coli NA