Reference - Detail
| RRC ID | 35286 |
|---|---|
| Author | Sinha J, Reyes SJ, Gallivan JP. |
| Title | Reprogramming bacteria to seek and destroy an herbicide. |
| Journal | Nat Chem Biol |
| Abstract |
A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this riboswitch can reprogram bacteria to migrate in the presence of atrazine. Finally, we show that incorporating a gene from an atrazine catabolic pathway allows these cells to seek and destroy atrazine. |
| Volume | 6(6) |
| Pages | 464-70 |
| Published | 2010-6-1 |
| DOI | 10.1038/nchembio.369 |
| PII | nchembio.369 |
| PMID | 20453864 |
| PMC | PMC2873063 |
| MeSH | Acetone / metabolism Atrazine / chemical synthesis Atrazine / metabolism* Atrazine / pharmacology Bacteria / drug effects Bacteria / genetics* Bacteria / metabolism* Bacterial Physiological Phenomena Base Sequence Cell Movement Cloning, Molecular DNA, Bacterial / genetics Ethanolamines / metabolism Gene Expression Regulation, Bacterial Herbicides / chemical synthesis Herbicides / metabolism* Kinetics Light Molecular Sequence Data RNA, Bacterial / chemistry RNA, Bacterial / genetics RNA, Bacterial / metabolism beta-Galactosidase / metabolism |
| IF | 12.587 |
| Times Cited | 127 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Prokaryotes E. coli | NA |