論文 - 詳細
| RRC ID | 35286 |
|---|---|
| 著者 | Sinha J, Reyes SJ, Gallivan JP. |
| タイトル | Reprogramming bacteria to seek and destroy an herbicide. |
| ジャーナル | Nat Chem Biol |
| Abstract |
A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this riboswitch can reprogram bacteria to migrate in the presence of atrazine. Finally, we show that incorporating a gene from an atrazine catabolic pathway allows these cells to seek and destroy atrazine. |
| 巻・号 | 6(6) |
| ページ | 464-70 |
| 公開日 | 2010-6-1 |
| DOI | 10.1038/nchembio.369 |
| PII | nchembio.369 |
| PMID | 20453864 |
| PMC | PMC2873063 |
| MeSH | Acetone / metabolism Atrazine / chemical synthesis Atrazine / metabolism* Atrazine / pharmacology Bacteria / drug effects Bacteria / genetics* Bacteria / metabolism* Bacterial Physiological Phenomena Base Sequence Cell Movement Cloning, Molecular DNA, Bacterial / genetics Ethanolamines / metabolism Gene Expression Regulation, Bacterial Herbicides / chemical synthesis Herbicides / metabolism* Kinetics Light Molecular Sequence Data RNA, Bacterial / chemistry RNA, Bacterial / genetics RNA, Bacterial / metabolism beta-Galactosidase / metabolism |
| IF | 12.587 |
| 引用数 | 127 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | NA |