論文 - 詳細
| RRC ID | 35287 |
|---|---|
| 著者 | Soboh B, Krüger S, Kuhns M, Pinske C, Lehmann A, Sawers RG. |
| タイトル | Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli. |
| ジャーナル | FEBS Lett |
| Abstract |
By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a ΔhybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile. |
| 巻・号 | 584(18) |
| ページ | 4109-14 |
| 公開日 | 2010-9-24 |
| DOI | 10.1016/j.febslet.2010.08.037 |
| PII | S0014-5793(10)00699-X |
| PMID | 20807532 |
| MeSH | Carboxyl and Carbamoyl Transferases / genetics Endopeptidases / genetics Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Hydrogenase / chemistry Hydrogenase / genetics Hydrogenase / metabolism* Oxygen / metabolism* |
| IF | 3.057 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW2961 pCA24N-hybD? |