RRC ID |
35287
|
著者 |
Soboh B, Krüger S, Kuhns M, Pinske C, Lehmann A, Sawers RG.
|
タイトル |
Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli.
|
ジャーナル |
FEBS Lett
|
Abstract |
By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a ΔhybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile.
|
巻・号 |
584(18)
|
ページ |
4109-14
|
公開日 |
2010-9-24
|
DOI |
10.1016/j.febslet.2010.08.037
|
PII |
S0014-5793(10)00699-X
|
PMID |
20807532
|
MeSH |
Carboxyl and Carbamoyl Transferases / genetics
Endopeptidases / genetics
Escherichia coli / enzymology*
Escherichia coli / genetics
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Hydrogenase / chemistry
Hydrogenase / genetics
Hydrogenase / metabolism*
Oxygen / metabolism*
|
IF |
3.057
|
引用数 |
10
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
JW2961
pCA24N-hybD? |