RRC ID 35287
著者 Soboh B, Krüger S, Kuhns M, Pinske C, Lehmann A, Sawers RG.
タイトル Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli.
ジャーナル FEBS Lett
Abstract By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a ΔhybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile.
巻・号 584(18)
ページ 4109-14
公開日 2010-9-24
DOI 10.1016/j.febslet.2010.08.037
PII S0014-5793(10)00699-X
PMID 20807532
MeSH Carboxyl and Carbamoyl Transferases / genetics Endopeptidases / genetics Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Hydrogenase / chemistry Hydrogenase / genetics Hydrogenase / metabolism* Oxygen / metabolism*
IF 3.057
引用数 10
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
原核生物(大腸菌) JW2961 pCA24N-hybD?