論文 - 詳細
| RRC ID | 35292 |
|---|---|
| 著者 | Uchiyama J, Sasaki Y, Nagahama H, Itou A, Matsuoka S, Matsumoto K, Hara H. |
| タイトル | Accumulation of sigmaS due to enhanced synthesis and decreased degradation in acidic phospholipid-deficient Escherichia coli cells. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
The Escherichia coli pgsA3 mutation, which causes deficiency in acidic phospholipids, leads to a significant accumulation of sigma(S). This accumulation is partly accounted for by the higher transcription level of rpoS; however, it has also been suggested that the cells accumulate sigma(S) post-transcriptionally. We found that the level of the small regulatory RNA RprA, which is involved in the promotion of rpoS translation, is higher in pgsA3 cells than in pgsA(+) cells. Induction of altered rpoS mRNA that does not depend on RprA in pgsA(+) cells did not increase the level of sigma(S) to the high level observed in pgsA3 cells, suggesting post-translational sigma(S) accumulation in the latter. The mRNA levels of clpX and clpP, whose products form a ClpXP protease that degrades sigma(S), were much reduced in pgsA3 cells. Consistent with the reduced mRNA levels, the half-life of sigma(S) in pgsA3 cells was much longer than in pgsA(+) cells, indicating that downregulation of the degradation is a major cause for the high sigma(S) content. We show that the downregulation can be partially attributed to activated CpxAR in the mutant cells, which causes repression of rpoE on whose gene product the expression of clpPX depends. |
| 巻・号 | 307(2) |
| ページ | 120-7 |
| 公開日 | 2010-6-1 |
| DOI | 10.1111/j.1574-6968.2010.01964.x |
| PII | FML1964 |
| PMID | 20455949 |
| MeSH | ATPases Associated with Diverse Cellular Activities Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism* Endopeptidase Clp / genetics Endopeptidase Clp / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Half-Life Molecular Chaperones / genetics Molecular Chaperones / metabolism Mutation Phospholipids / metabolism* Protein Kinases / genetics Protein Kinases / metabolism RNA, Bacterial RNA, Messenger Sigma Factor / genetics Sigma Factor / metabolism* Signal Transduction Transferases (Other Substituted Phosphate Groups) / genetics Transferases (Other Substituted Phosphate Groups) / metabolism |
| IF | 1.987 |
| 引用数 | 2 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | JW0438-KC JW3382-KC JW3383-KC |