論文 - 詳細
| RRC ID | 35297 |
|---|---|
| 著者 | Yosef I, Bochkareva ES, Bibi E. |
| タイトル | Escherichia coli SRP, its protein subunit Ffh, and the Ffh M domain are able to selectively limit membrane protein expression when overexpressed. |
| ジャーナル | mBio |
| Abstract |
The Escherichia coli signal recognition particle (SRP) system plays an important role in membrane protein biogenesis. Previous studies have suggested indirectly that in addition to its role during the targeting of ribosomes translating membrane proteins to translocons, the SRP might also have a quality control role in preventing premature synthesis of membrane proteins in the cytoplasm. This proposal was studied here using cells simultaneously overexpressing various membrane proteins and either SRP, the SRP protein Ffh, its 4.5S RNA, or the Ffh M domain. The results show that SRP, Ffh, and the M domain are all able to selectively inhibit the expression of membrane proteins. We observed no apparent changes in the steady-state mRNA levels or membrane protein stability, suggesting that inhibition may occur at the level of translation, possibly through the interaction between Ffh and ribosome-hydrophobic nascent chain complexes. Since E. coli SRP does not have a eukaryote-like translation arrest domain, we discuss other possible mechanisms by which this SRP might regulate membrane protein translation when overexpressed. |
| 巻・号 | 1(2) |
| 公開日 | 2010-6-8 |
| DOI | 10.1128/mBio.00020-10 |
| PII | e00020-10 |
| PMID | 20714446 |
| PMC | PMC2921155 |
| MeSH | Escherichia coli / chemistry Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial* Membrane Proteins / chemistry Membrane Proteins / genetics* Membrane Proteins / metabolism Protein Stability Protein Structure, Tertiary Protein Subunits / chemistry Protein Subunits / genetics Protein Subunits / metabolism Signal Recognition Particle / chemistry Signal Recognition Particle / genetics Signal Recognition Particle / metabolism* |
| IF | 6.784 |
| 引用数 | 12 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |