| RRC ID |
3532
|
| 著者 |
Paschinger K, Hackl M, Gutternigg M, Kretschmer-Lubich D, Stemmer U, Jantsch V, Lochnit G, Wilson IB.
|
| タイトル |
A deletion in the golgi alpha-mannosidase II gene of Caenorhabditis elegans results in unexpected non-wild-type N-glycan structures.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The processing of N-linked oligosaccharides by alpha-mannosidases in the endoplasmic reticulum and Golgi is a process conserved in plants and animals. After the transfer of a GlcNAc residue to Asn-bound Man(5)GlcNAc(2) by N-acetylglucosaminyltransferase I, an alpha-mannosidase (EC 3.2.1.114) removes one alpha1,3-linked and one alpha1,6-linked mannose residue. In this study, we have identified the relevant alpha-mannosidase II gene (aman-2; F58H1.1) from Caenorhabditis elegans and have detected its activity in both native and recombinant forms. For comparative studies, the two other cDNAs encoding class II mannosidases aman-1 (F55D10.1) and aman-3 (F48C1.1) were cloned; the corresponding enzymes are, respectively, a putative lysosomal alpha-mannosidase and a Co(II)-activated alpha-mannosidase. The analysis of the N-glycan structures of an aman-2 mutant strain demonstrates that the absence of alpha-mannosidase II activity results in a shift to structures not seen in wild-type worms (e.g. N-glycans with the composition Hex(5-7)HexNAc(2-3)Fuc(2)Me) and an accumulation of hybrid oligosaccharides. Paucimannosidic glycans are almost absent from aman-2 worms, indicative also of a general lack of alpha-mannosidase III activity. We hypothesize that there is a tremendous flexibility in the glycosylation pathway of C. elegans that does not impinge, under standard laboratory conditions, on the viability of worms with glycotypes very unlike the wild-type pattern.
|
| 巻・号 |
281(38)
|
| ページ |
28265-77
|
| 公開日 |
2006-9-22
|
| DOI |
10.1074/jbc.M602878200
|
| PII |
S0021-9258(19)58113-3
|
| PMID |
16864579
|
| PMC |
PMC2848328
|
| MeSH |
Animals
Caenorhabditis elegans / enzymology*
Glycosylation
Golgi Apparatus / enzymology*
Mutation
Polysaccharides / biosynthesis
Polysaccharides / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
alpha-Mannosidase / genetics
alpha-Mannosidase / physiology*
|
| IF |
4.238
|
| 引用数 |
32
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm1078 |
