RRC ID 35343
Author Ma Y, Yu H, Pan W, Liu C, Zhang S, Shen Z.
Title Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant.
Journal Bioresour. Technol.
Abstract Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative recombinant, R. ruber TH3 was constructed. Its nitrile hydratase activity was 25% higher than that of the wild type, reaching 490+/-29.2U/mg dry cell weight, while the amidase activity was 60% lower. After 6 h hydration of acrylonitrile using free cells as biocatalysts at 18 degrees C, acrylamide production by TH3 was 23% higher and the production of the by-product, acrylic acid, was 87% lower than that of the wild type. This result demonstrates that the strain TH3 could be valuable for industrial applications.
Volume 101(1)
Pages 285-91
Published 2010-1
DOI 10.1016/j.biortech.2009.07.057
PII S0960-8524(09)00911-0
PMID 19720524
MeSH Amidohydrolases / genetics Amidohydrolases / metabolism* Gene Knockout Techniques Hydro-Lyases / biosynthesis* Mutation / genetics Rhodococcus / genetics Rhodococcus / isolation & purification* Rhodococcus / metabolism* Species Specificity
IF 5.807
Times Cited 17
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS AGRICULTURAL ENGINEERING
Resource
Prokaryotes E. coli NA