論文 - 詳細
| RRC ID | 35343 |
|---|---|
| 著者 | Ma Y, Yu H, Pan W, Liu C, Zhang S, Shen Z. |
| タイトル | Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant. |
| ジャーナル | Bioresour Technol |
| Abstract |
Microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. During the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. A novel strain with high nitrile hydratase activity was isolated from soil and identified as Rhodococcus ruber TH by morphology and 16S rRNA gene analysis. An amidase-negative recombinant, R. ruber TH3 was constructed. Its nitrile hydratase activity was 25% higher than that of the wild type, reaching 490+/-29.2U/mg dry cell weight, while the amidase activity was 60% lower. After 6 h hydration of acrylonitrile using free cells as biocatalysts at 18 degrees C, acrylamide production by TH3 was 23% higher and the production of the by-product, acrylic acid, was 87% lower than that of the wild type. This result demonstrates that the strain TH3 could be valuable for industrial applications. |
| 巻・号 | 101(1) |
| ページ | 285-91 |
| 公開日 | 2010-1-1 |
| DOI | 10.1016/j.biortech.2009.07.057 |
| PII | S0960-8524(09)00911-0 |
| PMID | 19720524 |
| MeSH | Amidohydrolases / genetics Amidohydrolases / metabolism* Gene Knockout Techniques Hydro-Lyases / biosynthesis* Mutation / genetics Rhodococcus / genetics Rhodococcus / isolation & purification* Rhodococcus / metabolism* Species Specificity |
| IF | 7.539 |
| 引用数 | 32 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS AGRICULTURAL ENGINEERING |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | NA |