論文 - 詳細
| RRC ID | 35349 |
|---|---|
| 著者 | Chadani Y, Ono K, Kutsukake K, Abo T. |
| タイトル | Escherichia coli YaeJ protein mediates a novel ribosome-rescue pathway distinct from SsrA- and ArfA-mediated pathways. |
| ジャーナル | Mol Microbiol |
| Abstract |
Accumulation of stalled ribosomes at the 3' end of mRNA without a stop codon (non-stop mRNA) is supposed to be toxic to bacterial cells. Escherichia coli has at least two distinct systems to rescue such stalled ribosomes: SsrA-dependent trans-translation and ArfA-dependent ribosome rescue. Combination of the ssrA and arfA mutations is synthetically lethal, suggesting the significance of ribosome rescue. In this study, we identified the E. coli yaeJ gene, encoding a peptide-release factor homologue with GGQ motif, as a multicopy suppressor of the lethal phenotype of ssrA arfA double mutant. The YaeJ protein was shown to bind to ribosomes. Both in vivo and in vitro, YaeJ showed the ribosome-rescue activity and promoted the hydrolysis of peptidyl-tRNA residing in the stalled ribosome. Missense mutation in the GGQ motif or deletion of the C-terminal unstructured tail abolished both the suppressor activity for ssrA arfA synthetic lethality and the ribosome-rescue activity, suggesting the importance of these structural features. On the basis of these observations, we propose that YaeJ acts as a stop codon-independent peptidyl-tRNA hydrolysing factor through binding to ribosomes stalled at the 3' end of non-stop mRNAs. It was also suggested that ArfA and YaeJ rescue the stalled ribosomes by distinct mechanisms. |
| 巻・号 | 80(3) |
| ページ | 772-85 |
| 公開日 | 2011-5-1 |
| DOI | 10.1111/j.1365-2958.2011.07607.x |
| PMID | 21418110 |
| MeSH | Amino Acid Sequence Amino Acid Substitution / genetics Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism* Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Dosage Molecular Sequence Data Mutant Proteins / genetics Mutant Proteins / metabolism Mutation, Missense Protein Binding RNA, Transfer, Amino Acyl / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Ribosomes / metabolism* Sequence Alignment Sequence Deletion |
| IF | 3.418 |
| 引用数 | 76 |
| WOS 分野 | MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW0187-KC |