RRC ID 35358
著者 Goto S, Kato S, Kimura T, Muto A, Himeno H.
タイトル RsgA releases RbfA from 30S ribosome during a late stage of ribosome biosynthesis.
ジャーナル EMBO J
Abstract RsgA is a 30S ribosomal subunit-binding GTPase with an unknown function, shortage of which impairs maturation of the 30S subunit. We identified multiple gain-of-function mutants of Escherichia coli rbfA, the gene for a ribosome-binding factor, that suppress defects in growth and maturation of the 30S subunit of an rsgA-null strain. These mutations promote spontaneous release of RbfA from the 30S subunit, indicating that cellular disorders upon depletion of RsgA are due to prolonged retention of RbfA on the 30S subunit. We also found that RsgA enhances release of RbfA from the mature 30S subunit in a GTP-dependent manner but not from a precursor form of the 30S subunit. These findings indicate that the function of RsgA is to release RbfA from the 30S subunit during a late stage of ribosome biosynthesis. This is the first example of the action of a GTPase on the bacterial ribosome assembly described at the molecular level.
巻・号 30(1)
ページ 104-14
公開日 2011-1-5
DOI 10.1038/emboj.2010.291
PII emboj2010291
PMID 21102555
PMC PMC3020115
MeSH Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism* Mutation Protein Binding Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Ribosome Subunits, Small, Bacterial / metabolism* Ribosomes / metabolism*
IF 9.889
引用数 31
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
原核生物(大腸菌) ASKA library