RRC ID 35377
著者 Volokhina EB, Grijpstra J, Stork M, Schilders I, Tommassen J, Bos MP.
タイトル Role of the periplasmic chaperones Skp, SurA, and DegQ in outer membrane protein biogenesis in Neisseria meningitidis.
ジャーナル J Bacteriol
Abstract The periplasmic chaperones Skp, SurA, and DegP are implicated in the biogenesis of outer membrane proteins (OMPs) in Escherichia coli. Here, we investigated whether these chaperones exert similar functions in Neisseria meningitidis. Although N. meningitidis does not contain a homolog of the protease/chaperone DegP, it does possess a homolog of another E. coli protein, DegQ, which can functionally replace DegP when overproduced. Hence, we examined whether in N. meningitidis, DegQ acts as a functional homolog of DegP. Single skp, surA, and degQ mutants were easily obtained, showing that none of these chaperones is essential in N. meningitidis. Furthermore, all combinations of double mutants were generated and no synthetic lethality was observed. The absence of SurA or DegQ did not affect OMP biogenesis. In contrast, the absence of Skp resulted in severely lower levels of the porins PorA and PorB but not of other OMPs. These decreased levels were not due to proteolytic activity of DegQ, since porin levels remained low in a skp degQ double mutant, indicating that neisserial DegQ is not a functional homolog of E. coli DegP. The absence of Skp resulted in lower expression of the porB gene, as shown by using a P(porB)-lacZ fusion. We found no cross-species complementation when Skp of E. coli or N. meningitidis was heterologously expressed in skp mutants, indicating that Skp functions in a species-specific manner. Our results demonstrate an important role for Skp but not for SurA or DegQ in OMP biogenesis in N. meningitidis.
巻・号 193(7)
ページ 1612-21
公開日 2011-4-1
DOI 10.1128/JB.00532-10
PII JB.00532-10
PMID 21296967
PMC PMC3067652
MeSH Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Escherichia coli / metabolism Gene Expression Regulation, Bacterial / physiology* Molecular Chaperones / genetics Molecular Chaperones / metabolism* Mutation Neisseria meningitidis / genetics Neisseria meningitidis / metabolism* Porins
IF 3.006
引用数 45
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113) JW0173-KC