Reference - Detail
RRC ID | 35382 |
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Author | Miyoshi K, Matsuura H, Kumon T, Matsuda K, Yoshikawa T, Bamba T, Harada K, Hirata K. |
Title | Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase. |
Journal | J Biosci Bioeng |
Abstract |
We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported. |
Volume | 112(1) |
Pages | 75-8 |
Published | 2011-7-1 |
DOI | 10.1016/j.jbiosc.2011.02.012 |
PII | S1389-1723(11)00076-4 |
PMID | 21444242 |
MeSH | Amino Acid Sequence Chlamydomonas reinhardtii / genetics Chlamydomonas reinhardtii / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Molecular Sequence Data Phosphotransferases (Carboxyl Group Acceptor) / genetics Phosphotransferases (Carboxyl Group Acceptor) / isolation & purification* Phosphotransferases (Carboxyl Group Acceptor) / metabolism Proline / metabolism Recombinant Proteins / metabolism |
IF | 2.366 |
Times Cited | 0 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY FOOD SCIENCE & TECHNOLOGY |
Resource | |
Prokaryotes E. coli | NA |